医学毕业论文补阳还五汤对全脑缺血模型大鼠再灌不同时点皮层神经元钙浓度的影响

《医学毕业论文补阳还五汤对全脑缺血模型大鼠再灌不同时点皮层神经元钙浓度的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、补阳还五汤对全脑缺血模型大鼠再灌不同时点皮层神经元钙浓度的影响姓名：2014年6月25日补阳还五汤对全脑缺血模型大鼠再灌不同时点皮层神经元钙浓度的影响作者：关莉，丁胜元，罗铭，闫福曼，李小英，周乐全，罗荣敬【摘要】目的采用钙离子图像分析技术，研究补阳还五汤预干预对全脑缺血大鼠皮层神经元内Ca2+浓度的影响，探讨Ca2+信号异常参与缺血性神经元损伤的机制及补阳还五汤抗脑缺血的分子机制，为中药复方的研宄探索新的思路和方法。方法参照改良的Pulsinelli四血管闭塞法制备全脑缺血大鼠模型，缺血后的大鼠分别在存活2、12、24、48及72h后进行皮层神经细胞急性分离、负载荧光指示剂Fura-2/AM

2、,用钙离子成像系统检测单个神经细胞內Ca2+的浓度。结果补阳还五汤预干预除再灌12h吋点之外，可显著降低其他各吋点大脑皮层神经元内Ca2+浓度，对缺血再灌后的2次Ca2+堆积均表现明显的抑制作用。结论降低缺血再灌注不同时点皮层神经元内Ca2+浓度是补阳还五汤抗脑缺血的重要机制之一。【关键词】补阳还五汤；全脑缺血模型大鼠；再灌不同吋点；大脑皮层神经元；钙离子浓度EffectsofBuyanghuanwutangonCa2+messageofglobalcerebralcorticalneuronsofratswithischemiaandreperfusionatdifferenttimepoi

3、nts【Abstract】ObjectiveApplicationtheion-imageanalyticaltechniquetosimultaneouslystudytheeffectsofBuyanghuanwutangontheintracellularCa2+concentrationsoftheischemicrats’cerebralcorticalneurons，intensivelydiscussthemechanismthattheabnormalityoftheneuronalCa2+messagejoinsintheischemicneuronalinjuryandth

4、emolecularmechanismofcerebralanti-ischemiawithBuyanghuanwutang,andexplorenewideasandmethodsfortheresearchonthecomplexprescription.MethodsConsultedthemodifiedPulsinelli1stetra-vesselocclusiontopreparetheglobalcerebralischemicrats.Thcrat’scerebralcorticalcellwasacquiredintheacuteseparationmethod.Andth

5、eCa2+concentrationofasingleneuronwastestedbytheCa2+image-formingsystem.ResultsThepreinterventionbyBuyanghuanwutangcoulddroptheCa2+concentrationoftheglobalcerebralcorticalneuronsofratswithischemiaandreperfusionatalltimepointsexcept12h，andobviouslyinhibitthetwoCa2+accumulationsafterischemiaandreperfus

6、ion.Itwasoneofthemainmechanismsofthecerebralanti-ischemiawithBuyanghuanwutangtodroptheCa2+concentrationoftheglobalcerebralcorticalneuronsoftheratswithischemiaandreperfusionatdifferenttimepoints.ConclusionThepreinterventionbyBuyanghuanwutangwasofsignificanttothepreventionandtreatmentoftheischemicneur

7、onalinjury.【Keywords】Buyanghuanwutang;ratswithischemiaandreperfusion;globalcerebralcorticalneurons;differenttimepoints;Ca2+concentrations脑缺血在祖国医学屮属于“屮风”范畴，气虚血瘀是致病的重要因素，故治则应为益气活血［1］。补阳还五汤具有补气活血化瘀之功，临床用