胰岛素对子宫内膜癌化疗增敏的体外实验

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1、华中科技大学硕士学位论文胰岛素对子宫内膜癌化疗增敏的体外实验研究生：杜璇指导老师：王泽华教授汪宏波副教授中文摘要目的：观察胰岛素联合力朴素（脂质体紫杉醇）对子宫内膜癌Ishikawa和Hec-1A的抑制作用，并探讨其内在的分子机制。方法：Ishikawa，Hec-1A细胞经胰岛素和力朴素处理后，以MTT法检测细胞的增殖情况，判断两药的相互作用；以流式细胞仪检测细胞凋亡和周期；逆转录聚合酶链反应（RT-PCR）检测凋亡抑制因子survivin的表达。结果：力朴素（脂质体紫杉醇）联合胰岛素可抑制Ishikawa和Hec-1A细胞增殖，且随时间延长抑制作用越来越

2、显著，当两药合用72h时抑制率分别达到63.2%和50.2%，与单独力朴素（脂质体紫杉醇）组相比差异有显著性（P<0.01），显示两者有协同作用。经不同药物组作用后的Ishikawa细胞凋亡差异明显，周期阻滞于G1期；而Hec-1A细胞凋亡差异不明显，周期阻滞于G2/M期。经胰岛素联合力朴素作用后的Ishikawa和Hec-1A细胞表达survivin较力朴素单独作用时明显下降。结论：体外力朴素可抑制Ishikawa和Hec-1A细胞增殖并诱导其发生凋亡，并可使survivin基因的表达下调，与胰岛素联合应用时可显著增加其抑制作用，表明胰岛素具有明显的化疗

3、增敏效应。【关键词】胰岛素；力朴素；子宫内膜癌；Ishikawa细胞；Hec-1A细胞；survivin;凋亡;2华中科技大学硕士学位论文Theexperimentofchemo-sensitizationofinsulinonendometrialcancerinvitroAbstractObjectiveToinvestigatetheeffectsofadditionofinsulin,akindofchem-sensitizer,combinedwithpaclitaxelliposomeontheapoptosisofendometrialcan

4、cercelllinesIshikawaandHec-1Aandtoexplorethepossiblemolecularmechanisms.MethodsIshikawaandHec-1Acellsweretreatedwithinsulinand(or)paclitaxelliposomeinvitro.CellproliferationwasassessedbyMTTassay.Cellapoptosiswasdetectedbyflowcytometry(FCM).TheexpressionofsurvivininIshikawaandHec-1

5、Awasdetectedbyreversetranscription-polymerasechainreaction(RT-PCR).ResultsWhentreatedwithinsulincombinedwithpaclitaxelliposomefor72h, theproliferationinhibitionratewas63.2%forIshikawacellsand50.2%forHec-1A cells,whichwassignificantlyhigherthanthoseofthecellstreatedwithinsulinor pa

6、clitaxelliposomealone(p<0.01).TheapoptosisofIshikawaandHec-1Acellsinduced byinsulinandpaclitaxelliposomewassignificantlyhigherthanthatintheother groups.Thedifferenceofapoptosisbetweendifferentdrugteamswasveryobviousfor Ishikawacells,andthecyclewasblockedatG1;Whilethedifferenceofap

7、optosiswas notmarkedforHec-1Atreatedwithpaclitaxelliposomewithorwithoutinsulin,the cyclewasblockedatG2/M..TheexpressionofsurvivininIshikawaandHec-1Acells declinedobviouslyaftertreatmentofinsulincombinedwithpaclitaxelliposome. ConclusionPaclitaxelliposomewithadditionofinsulincoulds

8、ignificantly inhibiteproliferatio