血管新生与VEGF表达的影响

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1、华中科技大学硕士学位论文AbstractObjective:ToexploretheeffectsandpossiblemechanismofrecombinanthumanNeuregulin-1βoncardiacfunctionofacutemyocardialinfarction(AMI)rats,theangiogenesisandtheexpressionofvascularendothelialgrowthfactor(VEGF)inischemicmyocardium.Methods:

2、AMImodelwasestablishedwithSprague-Dawleyratsbyligationoftheleftanteriordescendingcoronaryartery．Theratswhichsurvived24hoursaftersurgerywererandomlyseparatedinto2groups:rhNrg-1βtreatmentgroupwithcaudal veininjectionofrhNrg-1βatadoseofl0µg·kg-1·d–1for5con

3、secutivedaysandthemyocardialinfarction(MI)controlgroup.Anothershamoperationgroupunderwentthoracicincisionwithoutligationofleftanteriordescendingcoronaryartery.MIcontrolgroupandshamoperationgroupweresimultaneouslyadministeredwith0.1mol/LPBS.Afterroutinef

4、eedandwaterfor28days,cardiacfunctionofdifferentgroupswereevaluatedbyechocardiography.Afterthat,theratsweresoonexecutedtodetecttheweightandmassindexofleftventricular.DensitiesofnewcollateralmicrovesselweremeasuredbyⅧfactorimmunohistochemicalanalysis.West

5、ernblottingwasadoptedtoobserveVEGFexpressionintheischemicmyocardiumofAMIrats.Results:ComparedwiththeMIcontrolgroup,cardiacfunctionoftherhNrg-1βtreatmentgroupobviouslyimproved;Comparedwiththeshamoperationgroup,alargenumberofnewbloodvesselsintheproliferat

6、ivestagecanbefoundintheinfarctionborderzoneofbothMIgroupandrhNrg-1βtreatmentgroup,andcapillarydensityintherhNrg-1βtreatmentgroupwassignificantlygreaterthanthatintheMIcontrolgroup;Besides,theexpressionofVEGFproteinsignificantlyincreasedintheinfarctionbor

7、derzoneofMIgroupandrhNrg-1βtreatmentgroup,andexpressionlevelintheMIgroupwasmuchlessthanthatintherhNrg-1βtreatmentgroup.Conclusions:rhNrg-1βincreaseexpressionofVEGFproteininischemicmyocardiumofAMIratswhichcanpromoteangiogenesisandcollateralcirculationtop

8、rotectischemicmyocardiumandimprovecardiacfunction.Keywords:rhNrg-1β；acutemyocardialinfarction；angiogenesis；VEGF3华中科技大学硕士学位论文英文缩略语简表Nrg-1βNeuregulin-1β神经调节蛋白-1β rhNrg-1βrecombinanthumanNeuregulin-1β重组人神经调节蛋白-1βAMIAcutemyocardialin