血管新生与VEGF表达的影响(6230)

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1、华中科技大学硕士学位论文AbstractObjective:ToexploretheeffectsandpossiblemechanismofrecombinanthumanNeuregulin-1βoncardiacfunctionofacutemyocardialinfarction(AMI)rats,theangiogenesisandtheexpressionofvascularendothelialgrowthfactor(VEGF)inischemicmyocardium.Methods:AMImodelwasestablishedwithSpr

2、ague-Dawleyratsbyligationoftheleftanteriordescendingcoronaryartery．Theratswhichsurvived24hoursaftersurgerywererandomlyseparatedinto2groups:rhNrg-1βtreatmentgroupwithcaudal veininjectionofrhNrg-1βatadoseofl0µg·kg-1·d–1for5consecutivedaysandthemyocardialinfarction(MI)controlgroup.Ano

3、thershamoperationgroupunderwentthoracicincisionwithoutligationofleftanteriordescendingcoronaryartery.MIcontrolgroupandshamoperationgroupweresimultaneouslyadministeredwith0.1mol/LPBS.Afterroutinefeedandwaterfor28days,cardiacfunctionofdifferentgroupswereevaluatedbyechocardiography.Af

4、terthat,theratsweresoonexecutedtodetecttheweightandmassindexofleftventricular.DensitiesofnewcollateralmicrovesselweremeasuredbyⅧfactorimmunohistochemicalanalysis.WesternblottingwasadoptedtoobserveVEGFexpressionintheischemicmyocardiumofAMIrats.Results:ComparedwiththeMIcontrolgroup,c

5、ardiacfunctionoftherhNrg-1βtreatmentgroupobviouslyimproved;Comparedwiththeshamoperationgroup,alargenumberofnewbloodvesselsintheproliferativestagecanbefoundintheinfarctionborderzoneofbothMIgroupandrhNrg-1βtreatmentgroup,andcapillarydensityintherhNrg-1βtreatmentgroupwassignificantlyg

6、reaterthanthatintheMIcontrolgroup;Besides,theexpressionofVEGFproteinsignificantlyincreasedintheinfarctionborderzoneofMIgroupandrhNrg-1βtreatmentgroup,andexpressionlevelintheMIgroupwasmuchlessthanthatintherhNrg-1βtreatmentgroup.Conclusions:rhNrg-1βincreaseexpressionofVEGFproteininis

7、chemicmyocardiumofAMIratswhichcanpromoteangiogenesisandcollateralcirculationtoprotectischemicmyocardiumandimprovecardiacfunction.Keywords:rhNrg-1β；acutemyocardialinfarction；angiogenesis；VEGF3华中科技大学硕士学位论文英文缩略语简表Nrg-1βNeuregulin-1β神经调节蛋白-1β rhNrg-1βrecombinanthumanNeuregulin-1β重组人神经调

8、节蛋白-1βAMIAcutemyocardialin