骨髓间充质干细胞静脉移植对损伤大鼠脊髓vegf、bfgf表达与血管新生的影响

《骨髓间充质干细胞静脉移植对损伤大鼠脊髓vegf、bfgf表达与血管新生的影响》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。

1、Reaction，RT—PCR)法检测VEGF和bFGFmRNA表达变化情况。6．4免疫组化检测第VIII因子相关抗原表达情况6．5采用Basso—Beattie—Bresnahan(BBB)评分和爬网格训练评估大鼠行为学改变。7．统计学分析结果：1．激光共聚焦显微镜显示脊髓损伤后经尾静脉移植的MSCs可达到脊髓损伤处聚集、存活且可以分泌生长因子VEGF和bFGF。2．RT—PCR结果显示VEGF和bFGFmRNA表达在损伤后比正常增高；移植后1天，MSCs移植组与对照组相比(P>0．05)，无统计学意义；移植后3天和14天，MSCs移植组均高于同一

2、时间段的对照组且随时间延长表达持续升高。3．第VIII因子相关抗原免疫组化结果显示：移植后1天，MSCs移植组与对照组相比(P>O．05)，无统计学意义；移植后3天和14天，MSCs移植组微血管密度(microvesseldensity，MVD)均高于同一时间段的对照组且随时间延长表达持续增多。4．BBB评分和爬网格训练显示移植MSCs可促进脊髓损伤大鼠后肢运动功能恢复。结论：静脉移植MSCs能在大鼠脊髓损伤区存活且分泌生长因子VEGF和bFGF，从而促进血管新生。关键词：骨髓间充质干细胞脊髓损伤静脉移植5Influenceofintravenous

3、administrationofbonemarrowmesenchymalstemcellsonexpressionofVEGF、bFGFandregenerationofbloodvesselafterspinalcordinjuryinratsAbstactObjective：ToobservethesurvivalandmigrationofBoneMarrowMesenchymalStemCel1SlabeledandthechangeonexpressionofVFGFandbfgffactorsatdifferenttimesaftert

4、ransplantionMSCviacaudalvientotreatcontusedspinalcordinjuriedadultSDratsmodel，andtoprovideexperimentalevidenceforthereparingfunctionandmenchanismwhichMSCstreatspinalcordinjury．Methods：1．Ratbonemarrowmesenchymalstemcells(MSCs)fromadultratswereculturedinvitro，theirspecificcellula

5、rsurfacemarkerwereanalysedwithflowcytometer．2．Experimentalanimalandgroups：42healthyadultsrague—dawely(SD)famalrats，meanweigh200—2509，wererandomlydividedinto7groupsincludingnormalcontrolgroup、controlgroupandMSCstransplantationgroups，eachgroups6rats．3．Establishcontusedspinalcordi

6、njuriedSDratmodel：ToadapttheAllen’Simprovedmethod一209weightironbarfallfrom5cmhighandstrikeatT12segmentspinalcordtogetcontusedspinalcordinjuriedratmodels．4．MSCareprelabeledwithCFSEinvitrobeforetransplantation．5．TransplantationofMSCs：InjectMSCsintothespinalcordinjurysiteafterinju

7、ry3daysintransplantgroups，butPBSareinjuriedincontusedcontrol6groups．6．MaterialSandEstimates6．1Heartperfusion、drawthespinalcordfromrat、slice-6．2ThesurviveofCFSE—MSCswhichweretransplantedintospinalcordinjuriedratsviaveinwasobservedbyusingconfocalmicroscopy6．3TheexpressionofVEGFan

8、dbFGFmRNAwasdetectedinspinalcordbyusingRT—PCR．6．4Immun