不同溺死类型大鼠肺组织中应激相关基因的表达水平分析

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1、-------山西医科大学硕士学位论文1.Amongthe6kindsofhousekeepinggenes,wasexcludedfromthestatisticswhichwasundetectedinmultiplesamples.B2M,HMBS,HPRT1,OAZ1,ACTBhadgoodstability,thedifferencetestbetweenthetwogroupsshowedallofPvaluesweremorethan0.05.2.88stressrelatedgenesexpressionwerenotsamewhencomparedbetweentwog

2、roups.Thereare25genesdifferentialexpressionwithalagermultiple(absolutevalue>2),theywereHRH1、JUN、CCL2、HSPB7、HDAC5、HDAC6、CXCL2、HSPB2、EP300、CCR4、CXCL10、CCL7、CCL5、STAC、HDAC4、MAP2K7、CIDEB、IL9、IL13、IL5、IL1A、CD40LG、HSPB3、SCARA5、FOS.Thereare13genesexpressionhadstatisticallysignificantdifference,theywereC

3、CL2、CECPB、IL1A、IL13、CD49LG、MAPK1、IL1B、LIG1、CCL7、TAF1、DGKK、CXCL1、CCR2.3.Put13geneswhichdifferentialexpressionwerestatisticallysignificant(P<0.1)intodatabasefortheGOtermenrichmentanalysis,itshowedthatthemainfunctionofthe13genesembodiedinfouraspects:thefirstOneistheregulationoftheactivityofcytokines

4、,immuneresponseanddamageresponse;theSecondonewasapositiveregulatorofthesynthesisofthesynthesisofmacromoleculesandmacromolecularpositivelyregulatesmetabolismprocess;Thethirdonewastoregulatecellproliferation;TheFourthonewasregulationofextracellularspace.Conclusion：1.Inthisexperiment,theselectedrefe

5、rencegenestabilitywasgood,whichwasconsistentwiththeourpreviousexperimentalresults.2.88stressrelatedgenesexpressionwerenotsamewhencomparedbetweentwogroups.Thereare25genesdifferentialexpressionwithalagermultiple(absolutevalue>2),thereare13genesexpressionhadstatisticallysignificantdifference(P＜0.1).

6、Theseshowedthattwokindsofdeathprocessinatypicaldrowningandtypicaldrowninghadthedifferentstressreactions.3.TheexpressionofCCLfamilygenesindeadratslungtissueshadsignificantdifferencesbetweentypicaldrowninggroupandatypicaldrowninggroup,especiallythe-----------VI-----------山西医科大学硕士学位论文Ccl2.Thisprompt

7、edustointhenextexperiment,specialattentionshouldbepaidtootherstressgeneresearchwithinthestressgenelibraryandCCLfamilygenes.Weshouldexpandtheanimalresearchsample,andfinalresearchtheapplicationsofstressrelatedgenesinhuma