细辛配伍附子含药血清对大鼠心肌细胞钠离子通道的影响

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1、成都中医药大学硕士学位论文AbstractObjective:ResearchingthecompatibilityofAsarumandAconitemedicatedserumonratmyocardialcellsandinfluenceoncell.Nachannel.Fortheapplicationof“xiangxuxiangshi”fortraditionalChinesemedicineprovidesomescientificbasisandprovethattheCompatibilityofAsarumandaconitecanmoretreatedforslow-t

2、ypearrhythmia.Methods:Cardiacmusclecellprimarycultureappliedtheexperiment.Thecellforexperimenthadtoculturefor3-5days.Andthecardiacmusclecellthatshouldhavefavorableselectionforlightscanbeprovidedforthecellpatch-clamptechniques.EffectofAsarumandaconitemedicatedserumrelatedpeptidesontheNachannelcurren

3、tbythewholecellpatch-clamptechniques.ThecurrentsweredigitizeddatawererecordedbyaLenovo-compatiblepersonalcomputerrunningpClamp10.0softwareandfurtheranalyzedbyClampfitprogram.WederivesexperimentprogramfromthefeaturesoftheNachannel.Thendrawingcurrentgraphofactivationcurveandrecoverycurve.Analysisingt

4、hecompatibilityofAsarumandaconitemedicatedserumonratmyocardialcellsandinfluenceoncellofNachannel.Results:(1)TheNachannel.characteristicsofprimaryculturecardiacmusclecell:Theinwardcurrentswerevoltage-dependent.Sodiumcurrentsweweactivatedat—60mV~—50mV,thenthecurrentsreachedthepeak.Withthevoltageincre

5、asing,thesodiumcurrentsdecreasegradually.At—10mV,thesodiumcurrentweredisappeared.Themembranecapacitanceofmyocardialcells(Rc)of－45.49±4.12pF.Thesodiumcurrentdensityof4.26±0.24pA/pF（n=16）.(2)theAsarumandAconiteherbsofmedicatedserumcanactivatedtheNachannel.ItLoweredthresholdpotential.Italsoeffecttheve

6、locityoftheactivation.ItcanshortenthetimeformyocardialcellsNachannelrecoverying.Itcandecurtat2成都中医药大学硕士学位论文theeffectiverefractoryperiod.Itenhancedrhythmicityofmyocardialcellscontraction.ThecompatibilityofAsarumandAconitemedicatedserumhavebetterefficiencythanusedalone.(3)TotheactivationofNachannel.T

7、houghtheserumcontaininginthetraditiongaldecoctionsolutionofAsarumisinferiortothatintheextratedvolatileoil,itseffectlastlong.Conclusions:ThecompatibilityofAsarumandAconitemedicatedserumhavebetterefficiencyth