清热化瘀颗粒对脑缺血预处理大鼠GRP78、PERK蛋白表达的影响

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1、目录中文摘要···················································2英文摘要···················································4引言····················································7正文····················································81.实验材料和方法·········································81.1实验对象

2、·················································81.2动物饲养条件·············································81.3主要试剂和药品···········································82.实验结果···············································133.讨论················································154.存在的问题和展望····

3、··································23结论··················································26参考文献··················································27附录··················································31综述··················································34致谢··························

4、························42攻读学位期间发表的学术论文目录·······················43作者声明··················································44个人简历··················································451缩略词BIPbrainischemicpreconditioning脑缺血预处理ERendoplasmiereticulum内质网ERSendoplasmicreticulumstress内质网应激ERSe

5、ndoplasmicreticulumstress脑缺血预处理GRP78glucoseregulatedprotein78葡萄糖调节蛋白78PERKPKR-likeERldnaseRNA依赖蛋白激酶内质网类似激酶I/Rischmia/reperfusion缺血再灌注ITischemictolerance缺血耐受TIAtransientischemicattack短暂性脑缺血发作UPRunfoldedproteinresponse,未折叠蛋白反应中文摘要目的：观察清热化瘀颗粒对局灶性脑缺血预处理大鼠前凋亡分子PERK蛋白及前存活分子GRP78蛋白表达的影响，并

6、探讨清热化瘀颗粒对预处理后二者在诱导脑缺血耐受中的意义。方法：SD大鼠随机分为4组每组20只，其中预缺血+脑缺血组（BIP组）与清热化瘀颗粒组（QRHY组）予预处理10min后抽出栓线，饲养3d，3d内BIP组、QRHY组分别给予等体积生理盐水和清热化瘀颗粒灌胃，每日2次，3d后再次行MCAO2h；假手术组（SO组）以假手术代替预缺血，假手术+脑缺血组（SO+MCAO组）假手术代替预缺血，其余步骤同脑缺血预处理组；采用Longa评分标准做神经行为学测定。采用HE染色观察各组脑组织病理形态。采用免疫组织化学比较各组PERK蛋白和GRP78蛋白的表达。结果：○1

7、神经行为学评分结果：除SO组外，各组均有神经行为学改变。SO+MCAO组较BIP组及QRHY组评分有显著差异，P＜0.01；BIP组较QRHY组评分有显著差异，（P<0.01）。○2MCAO2h后，各组脑组织形态学改变结果：在光镜下SO组脑组织神经元及胶质细胞基本正常；SO+MCAO组、BIP组梗死侧脑组织见典型缺血损伤改变；QRHY组较SO+MCAO组及BIP组病理切片结果有不同程度改善，QRHY组可见少许正常神经元。○3PERK与GRP78蛋白表达免疫组化结果：SO组，各时段均有极少PERK及GRP78蛋白表达；SO+MCAO组12h、1d、2d，PER

8、K和GRP78蛋白表达均高于假手术组，（P<0.01