去分化间充质干细胞再成骨分化潜能分析

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1、-目录研究背景··························································································1参考文献····················································································5第一部分：去分化间充质干细胞生物学特性··············································9材料与方法··

2、··············································································10结果······················································································12讨论······················································································14

3、参考文献···················································································16第二部分：去分化间充质干细胞体外成骨潜能分析····································17材料与方法················································································18结果······················

4、································································22第三部分：去分化间充质干细胞体内成骨潜能分析····································26材料与方法················································································27结果·············································

5、·········································31讨论······················································································34参考文献···················································································36结论和展望·································

6、·····················································41硕士研究生期间完成的论文·································································42中英文对照缩略词表··········································································43致谢·······································

7、·····················································44--------细菌纤维素的生物相容性及联合BMP2的成骨效应研究背景研究背景间充质干细胞(Mesenchymalstemcells,MSCs)来源于发育早期的中胚层和外胚层，最初在骨髓中被Friendenstein等发现为非造血组织干细胞，由于其具有可分化为间充质组织细胞的能力，故称之为间充质干细胞[1]。以后陆续在许多组织中分离到，包括脂肪、滑膜、软骨、骨膜、胎盘和脐血[2]。1.1间充质干细胞生物学特

8、性间充质干细胞具备以下共同属性：(1)，间充质干细胞是具有自我维持和自我更新的细胞群体，其细胞数目是相对恒定的，即间充质干细胞具有自稳定性。(2)，在生物体总的细胞构成比上，间充质干细胞只占有其中很小的一部分。(3)，间充质干细胞可在适宜环境中向不同方向分化，即其为相对未分化类细胞[3]。1，MSCs具有强大的自我更新和多向分化能力。可以分化为软骨细胞、成骨