资源描述：
《神经生长因子联合神经干细胞移植治疗大鼠脊髓损伤》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、中国组织工程研究与临床康复第14卷第14期2010–04–02出版JournalofClinicalRehabilitativeTissueEngineeringResearchApril2,2010Vol.14,No.14神经生长因子联合神经干细胞移植治疗大鼠脊髓损伤★范广明1，张文彬2，张赛2，王丽君3NervegrowthfactorcombinedwithneuralstemcelltransplantationfortreatingspinalcordinjuryinratsFanGuang-ming1,ZhangWen-bi
2、n2,ZhangSai2,WangLi-jun3Abstract1TianjinMedicalUniversity,TianjinBACKGROUND:Theeffectsofsimpleneuralstemcell(NSC)transplantationonrepairofdamagedspinalcordarenotideal.300070,China;Previousstudieshaveshownthatnervegrowthfactor(NGF)hasbotheffectsofneuronnutritionandprocess
3、growthpromotion,2AffiliatedHospitalofcaneffectivelycontributetotherecoveryofneurofunctionfollowingspinalcordinjury(SCI).MedicalCollegeofChinesePeople’sOBJECTIVE:ToinvestigatetheeffectofneuralstemcelltransplantationcombinedwithNGFapplicationontherecoveryofmotorArmedPolice
4、Force,functionofratswithSCI.Tianjin300162,METHODS:Atotalof42SpragueDawleyratswereusedtoestablishSPImodels,andthendividedrandomlyintothreegroups:China;3FirstPeople’smedium,simpleNSCsorNSCs+NGF.At1weekfollowinginjury,medium,simpleNSCsorNSCs+NGFwereseparatelyinjectedHospita
5、l,Lingyuan122500,Liaoningintodamagedsites.At1,2,4,6,8weekspost-injury,allanimalswereevaluatedonthehindlimbbehaviorwithBasso,BeattieandProvince,ChinaBresnahan(BBB)locomotorratingscaleandinclinedplanetest.At4weekspost-transplantation,histopathologyhematoxylin-eosinstaining
6、andBrdUimmunohistochemistrywereperformed.At8weekspost-transplantation,horseradishFanGuang-ming★,peroxidase(HRP)nervetraceandsomatosensoryevokedpotentialtestingwereperformedtoobservetherecoveryofnerveStudyingformaster’sdegree,Attendingelectrophysiology.physician,TianjinRE
7、SULTSANDCONCLUSION:At4weeksfollowinginjury,motorfunctionoftherathindlimbwassignificantlyimprovedintheMedicalUniversity,simpleNSCsgroupandNSCs+NGFgroup.TherecoverywasrapidintheNSCs+NGFgroupthanthesimpleNSCsgroup(P8、athologicalsectionsdemonstratedthatneuritewasnotfoundinthemediumChinaPhoenix2a2@163.group.Afewneuritesw
