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1、宁夏医科大学硕士研究生学位论文英文摘要EffectofpropofolonS100βProteincontent,totalcalciumcontent,watercontent,andthebraintissuemorphologyofacutebraininjuryinratsABSTRSCTObjective:ObservationofpropofolonacutebraininjuryinratsaccordingtoserumS100βProtein,braintissuetotalcalciumcontent,andwatercontent,andthechange
2、ofbraintissuemorphology.Explorepropofoleffectonacutebraininjuryinrats.Methods:84healthymaleSDratsarerandomlydividedintosixgroupswith14ratseach(n=14),shamgroup(groupS),braininjurygroup(groupI),fatemulsiongroup(groupF),Low-dosepropofolgroup(groupL),Middle-dosepropofolgroup(groupM)andHigh-dosep
3、ropofolgroup(groupH).ByimprovedFeeneyusingfreefallbraininjuryunit,establishedbraininjurymodelinrats:SDratsareused10%chloralhydrateto350mg/kgareanesthetizedbyintraperitonealinjection.Waituntildisappearofrightingreflex,eyelashreflexarepromptedanesthesiasuccess.Theratsarefixedsupinepositiononth
4、eoperatingtable,endotrachealintubation,connectsmallanimalventilation,tidalvolume2-3ml/100g,respiratoryrate50-60beats/min,inspiratorytoexpiratoryratio1:2.Separatingtheleftfemoralveinandcatheterforintravenousdrugs.Separatingtherightcommoncarotidarteryandcatheterforbloodsampling.Afterarterialan
5、dvenousareinserted,ratsareflxedforproneposition.Withpovidone-iodinedisinfectionoftheskin.Asepticoperation.Medianincision.Periostealstripping.Exposedtotherightparietalbone.Withadentaldrillafterthecoronalsuture1.5mm,midline2.5mmdrilleda5mmdiameterbonewindow,keepingintactdura.Gasketisplacedonth
6、ebonewindow,30cmheightwith20gweightfreefallalongtheIV万方数据宁夏医科大学硕士研究生学位论文英文摘要casing,resultinginrightparietalbraindamage,strikingforce600g·cm.Afterbeinghitbonewindowclosedwithbonewax,thescalpsutured.Shamgroup(groupS)openonlyafterbonewindowclosedwithbonewax,nottoimplementhit,theleftfemoralveini
7、sinfusedat3.49ml•kg-1•h-1continuousinfusedtheequalvolumeof0.9%saline60miningroupS.Braininjurygroup(groupI):Aftersuccessfulpreparationofbraininjurymodel,theleftfemoralveinisinfusedat3.49ml•kg-1•h-1continuousinfusedtheequalvolumeof0.9%saline60miningr
