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1、宁夏医科大学硕士研究生学位论文英文摘要EffectofpropofolonS100βProteincontent,totalcalciumcontent,watercontent,andthebraintissuemorphologyofacutebraininjuryinratsABSTRSCTObjective:ObservationofpropofolonacutebraininjuryinratsaccordingtoserumS100βProtein,braintissuetotalcalciumcontent,andwat
2、ercontent,andthechangeofbraintissuemorphology.Explorepropofoleffectonacutebraininjuryinrats.Methods:84healthymaleSDratsarerandomlydividedintosixgroupswith14ratseach(n=14),shamgroup(groupS),braininjurygroup(groupI),fatemulsiongroup(groupF),Low-dosepropofolgroup(groupL),M
3、iddle-dosepropofolgroup(groupM)andHigh-dosepropofolgroup(groupH).ByimprovedFeeneyusingfreefallbraininjuryunit,establishedbraininjurymodelinrats:SDratsareused10%chloralhydrateto350mg/kgareanesthetizedbyintraperitonealinjection.Waituntildisappearofrightingreflex,eyelashre
4、flexarepromptedanesthesiasuccess.Theratsarefixedsupinepositionontheoperatingtable,endotrachealintubation,connectsmallanimalventilation,tidalvolume2-3ml/100g,respiratoryrate50-60beats/min,inspiratorytoexpiratoryratio1:2.Separatingtheleftfemoralveinandcatheterforintraveno
5、usdrugs.Separatingtherightcommoncarotidarteryandcatheterforbloodsampling.Afterarterialandvenousareinserted,ratsareflxedforproneposition.Withpovidone-iodinedisinfectionoftheskin.Asepticoperation.Medianincision.Periostealstripping.Exposedtotherightparietalbone.Withadental
6、drillafterthecoronalsuture1.5mm,midline2.5mmdrilleda5mmdiameterbonewindow,keepingintactdura.Gasketisplacedonthebonewindow,30cmheightwith20gweightfreefallalongtheIV万方数据宁夏医科大学硕士研究生学位论文英文摘要casing,resultinginrightparietalbraindamage,strikingforce600g·cm.Afterbeinghitbonewin
7、dowclosedwithbonewax,thescalpsutured.Shamgroup(groupS)openonlyafterbonewindowclosedwithbonewax,nottoimplementhit,theleftfemoralveinisinfusedat3.49ml•kg-1•h-1continuousinfusedtheequalvolumeof0.9%saline60miningroupS.Braininjurygroup(groupI):Aftersuccessfulpreparationofbra
8、ininjurymodel,theleftfemoralveinisinfusedat3.49ml•kg-1•h-1continuousinfusedtheequalvolumeof0.9%saline60miningr
