人脐血干细胞移植治疗实验性自身免疫性脑脊髓炎的研究

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1、KKME---专业医学搜索引擎http://www.kkme.net/人脐血干细胞移植治疗实验性自身免疫性脑脊髓炎的研究作者：潘凤华，丁海霞，丁新生，王辉，张智弘作者单位：210011南京医科大学第二附属医院神经内科【关键词】人脐血干细胞;移植;实验性自身免疫性脑脊髓炎StudyonhumanumbilicalcordbloodcellstransplantationfortreatmentofexperimentalautoimmuneencephalomyelitisPANFenghua,D

2、INGHaixia,DINGXinsheng,etal.DepartmentofNeurology,theSecondAffiliatedHospitalofNanjingMedicalUniversity,Nanjing210011,ChinaAbstract：ObjectiveToexploretheeffectsofhumanumbilicalcordbloodcells(HUCBCs)transplantationtotreatexperimentalautoimmuneencepha

3、lomyelitis(EAE)ratsandthestatusoftransplantedHUCBCsinthebrainandspinalcordtissueofEAErats.MethodsThemononuclearcellsabstractedfromcordbloodofinfantswereculturedinvitroandmarkedwith5bromodeoxyuridine(Brdu)for48h.EAEratmodelsweremadeandtheHUCBCs(3×106)

4、weretransplantedintothetailvein(transplantedgroup)14dlater.Thescoreofneurologicalfunctiondificitandthenumberofthedemyelinatedfociinbrainandspinalcordwereundertakenatdifferenttimepointaftertransplantation.KKME---专业医学搜索引擎http://www.kkme.net/Thestatueofs

5、urvival,differentiationandmigrationofHUCBCsinvivoweredeterminedbyimmunohistochemicaltechnique,andcomparedwithcontrolgroup.ResultsThescoresofneurologicalfunctiondificitat21d,28dposttransplantationintransplantedgroupweremuchlowerthanthoseinthecontrolgro

6、up(allP<0.05).Withinthebrainandspinalcordtissues,BrdureactiveHUCBCsweredistributedthroughoutthelesionsateverytimepointaftertransplantationandweredifferentiatedintoastrocytes,oligodendrocytesandneuronsinvivo14d,later.MoreBrdureactivecellswerefoundinthe

7、brainandspinalcordtissuesat21d,28dthanthoseat7dand14d(allP<0.05).Comparedwithcontrolgroup,thenumberofdemyelinatedfocidecreasedsignificantlyat14daftertransplantation(allP<0.05).ConclusionHUCBCstransplantationcaneffectivelyimprovetheneurologicfunctionof

8、EAErats，theHUCBCsmaysurvive,migrateanddifferentiateinthebrainandspinalcordtissuesofEAErats,reducedemyelinatedfocis.Keywords：humanumbilicalcordbloodcells;transplantation;experimentalautoimmuneencephalomyelitisKKME---专业医学搜索引擎http://www.kkme.net/