外源性硫化氢对百草枯致大鼠急性肺损伤细胞炎性因子的调节作用

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1、Exogenoushydrogensulfideregulatestheexpressionofinflammatorycytolinesinthemodelofrat’sacutelunginjurywascausedbyparaquatAbstractPurpose：BymeasuringthecontentofIL-10、IL-1β、ICAM-1andNF-κBtoobservethemechanismofH2Splayaprotectiononlungthroughadjustinflammatorycytokinesandhopingtoprovidet

2、hetheoreticalbasisforclinicaltreatmentofparaquatpoisoning.Methods：Sixtyhealthyratswererandomlydividedinto6groups.Theblankgroup：NaClgroup（intraperitonealinjectionof0.9%NaCl）、TheNaHSgroup(intraperitonealinjectionofNaHS);Experimentalgroups:PQgroup、PQ+lowdoseofNaHS（Lgroup0.78mg/kg）、PQ+mid

3、dledoseofNaHS（Mgroup1.56mg/kg）、PQ+highdoseofNaHS（Hgroup3.12mg/kg）.AllratswerebleededtodeathafterPQlavaged24hours；Observingthelungwet/dryweight，rightlungtissuestainedwithHE，athighmagnificationobservedthechangesofpathologicalscoreoflunginjury.Threeexperimentalanalysismethods(WesternBlot

4、、ELISAandRT-PCR)wererespectivelyusedtomeasuretheexpressionofNF-κBproteininthelungtissue、thecontentofIL-1βandIL-10intheplasmaofrats、thelevelofIL-1β、IL-10andICAM-1mRNAinthelungtissueResults:1.Generalactivityinrats、lungtissuepathologychangeand(IQA)score：AttheopticalmicroscopeobservedtheH

5、Estainingoflungtissuesection，betweenNaClgroupandNaHSgroupdidnotseethepathologicalchange,IQAhadnotobviousdifference（P＞0.5）.ThelungtissuestructureofPQgroupwasobviouslydamaged，therewerealargenumberofalveolarintervalfractureandmutualfusion,manyredbloodcellsandinflammatorycellsinthealveola

6、r,meanwhilealveolarseptumvisibleinflammatorycellinfiltrationandseptainlungtissuechangedintothickening,someofalveolartransparentmembraneformation,IQAand（W/D）inPQgroupwasincreasedhadstatisticallysignificantly(P＜0.01).InL、MandHgroupsmostofstructureofalveolarwereintactandIQA、（W/D）wasstati

7、sticallysignificantlylowerthanPQgroup(P＜0.01)andthelevelofdecreasewaspositivelycorrelatedwiththeconcentrationofH2S,butcomparewiththeNaClandNaHSgroups，Therewerestillobviouslydamaged.万方数据1.ThecontentofIL-10、IL-1β、ICAM-1、NF-κBComparedwithNaClgrouprats,NaHSgroupratsthecontentofIL-1β、IL-10

8、inthe