棉籽蛋白和多糖提取工艺的分析

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1、山东农业大学硕士学位论文StudiesontheExtractionofCottonseedProteinandStudies0ntheExtractionotottonseedProtemandPolysaccharideAbstractCottonisakindofmajoragriculturalproductinourcountry．Cottonseedasancomponentofitisnotonlyanimportantplantoil，butalsoanimportantsourceofplantprote

2、in，secondonlytosoybeanintheworld．Aftertheoilextractedandgossypolremoved，therewasahighproteincontentinthecottonseedmeal，usuallybeing33．21％～45．09％．Cottonseedproteinwithoutbeanyflavorwillnotmakepeoplefeelflatulentaftereating．Soitisabetterplantproteinthansoy’S，worthof

3、developingandutilizinginfoodandfeedresources．Thephenolandfat-removedcottonseedasfewmaterialtoextractcottonseedprotein，usesthemethodofalkaliextractionandacidprecipitation，determiningtheextractionprocessconditionsanddifferentdecolorizationmethods．Andafterstudyingthe

4、thermalstability,waterholdingcapacity,oilabsorptionabilityandotherphysicalandchemicalpropetiesofcottonseedprotein，thealkali-extractionconditionsofthepolysaccharidesincottonseedmealareconfirmed．Theresultsareasfollows：1．Theoptimumconditionsofalkali-extractionofcotto

5、nseedproteinweredeterminedasfollows：thepHwas10，thetemperaturewas50"{2，thesolid-liquidratiowas1：20，thetimeWas90min,thecrushingmeshoftherawmaterialwere100andtheextractiontimeswere2．ChoosingthreemainfactorsincludingpH，temperatureandsolid-liquidratioforresponsesurface

6、experiments，theresultswereasfollows：thepHwas10．5，thetemperaturewas47．5"Candthesolid-liquidratioWas1：20(rn／v)．Underaboveconditions，theextractionrateofalkali-solublecottonseedproteinWasupto53．78％．2．Cottonseedproteincontainstwokinds，whicharestorageproteinandnon-stora

7、geprotein．ThemaxinlulnamountofproteinprecipitationWas5．21mginnon-storageproteinatpH4．5，while4．85mginstorageproteinatpH6．3．BySDS-PAGEdeterminescottonseedproteininthesupematantonmolecularweightof22．30，20．80，14．20kDa；Precipitationoftheproteinmolecularweightwere53．70k

8、Da,b50．10kD，alkalisolubleacidsinkingmethodCaneffectivelyseparatethehighmolecularweightandlowmolecularweightprotein．4．Twodifferentdecolorizationmethods，u