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1、宁波大学硕士专业学位论文ThestudyonPhasolosmaesculentapolysaccharidesinmiceimmunomodulatoryAbstractPhascolosomaesculenta(Phasolosmaesculenta),commonlyknownastheHedin,wormleech,sealeech,etc.Asournativespecies,mainlydistributedintheEastSouthChinaSea,benthicburrowinglife.AnumberofCh
2、inesePharmacopoeiarecordedesculentaedibleandmedicinalvalue,insomeareasevensubstituteforthetraditionalChinesemedicineCordycepssinensis.Esculentawithnourishingyin,kidneyfunctions,regularconsumptioncanenhancethebodyresistivity.Usingitasrawmaterial,“shootsfrozensoil”isal
3、sooneofourcountrycoastalpeoplefavoritefoods.AtpresentaboutPhascolosomaesculentahasbeguntotakeshape,butitsdevelopmentandutilizationdegreeislow,onlyconsumptionasanourishingfood,thepotentialmedicalvaluehasnotyetbeendeveloped.Polysaccharideisacombinationofmonosacchatidem
4、oleculardehydrationg,widelyfoundinthenature,istheimportantbioactivesubstance.Polysaccharidewidelyinvolvedinsubstancetransportation,immuneregulation,cellidentificationandotheractivitiesofthebody.Inthisthesis,thePhasolosmaesculentapolysaccharide(Phasolosmaesculentapoly
5、saccharide,PEP)astheresearchobject,throughtheextractionprocessoptimizationofactivepolysaccharidesandthecomponentfunctionofPEPtesttoevaluatetheanti-sepsis,studiestheimmunefunctionofactivepolysaccharidesonmiceadjustmentfunction.StudiesontheantioxidantactivityofPhasolos
6、maesculentapolysaccharidesextractionprocess.Onthebasisofsingle-factor,thesinglefactorconditionDPPH•scavengingactivityasanindex,todeterminetheextractiontime,theamountofenzymeextracttheoptimumrangeoftemperature.Throughtheresponsesurfaceoptimizationanalysis,optimization
7、andgettheoptimalextractionconditions.Theresultsshowedthatoptimalextractionconditionswere65℃,7hand10000U/g.Undertheseconditions,theexperimentalyieldsofpolysaccharideswas0.879±0.001%,whileDPPH·scavengingactivitywas72.5%.UndertheoptimumconditionobtainedtheactivePEP,anal
8、ysisitscomponentandidentificatesitsstructure.Phascolosomaesculentaenzymesolution,TCA(trichloroaceticacid)methodofremovingprotein,by
