微小rna与子宫内膜异位症患者卵子胚胎发育潜能的相关性研究

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1、授予单位代码10089学号或申请号20153078中国图书分类号R711.6HebeiMedicalUniversity硕士学位论文专业学位微小RNA与子宫内膜异位症患者卵子胚胎发育潜能的相关性研究研究生：袁巨芳导师：赵志明教授专业：妇产科学二级学院：河北医科大学第二医院2018年3月目录中文摘要············································································································1英文摘要···································

2、·········································································2英文缩写············································································································4研究论文微小RNA与子宫内膜异位症患者卵子胚胎发育潜能的相关性研究前言···········································································

3、·································6材料与方法································································································7结果··········································································································15附图·················································

4、·························································18附表··········································································································22讨论··········································································································30结论················

5、··························································································32参考文献··································································································32综述微小RNA与女性生殖功能相关性研究······································35致谢········································

6、··········································································44个人简历··········································································································45中文摘要微小RNA与子宫内膜异位症患者卵子胚胎发育潜能的相关性研究摘要目的:探讨miRNA对EMs的预测、对IVF-ET胚胎发育潜能和结局的影响。方法：收集2017年3月至2017年12月于我中心行IVF-ET的EMs

7、患者36例（实验组，A组）的血清、卵泡液及颗粒细胞，以同期单纯因输卵管不孕患者36例（对照组，B组）作为对照组。RT-PCR法检测A组和B组血清、卵泡液、颗粒细胞中miRNA的表达。结果：（1）血清、卵泡液、颗粒细胞中miR-142-3p、miR-21与年龄、不孕年限、基础FSH水平、COS天数、Gn用量及HCG日激素水平（LH、E2、P）无相关性。（2）血清、卵泡液中miR-142-3p、miR-21表达水平，A组均明显高于B组，