人参稀有皂苷compound+k的酶法制备及其混合胶束的研究

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1、万方数据摘要一anlecithinasthecarrier,Byingsolventevaporationmethodtopreparemixedmicelles，theorthogona-1designisusedtoscreenthebestpreparationtechnology；Investigatingmorphology,particlesize，coatingrate，drug-polymerinteractions，invitroreleasedegree；Andthrought

2、heCaco-2cellmodeltoevaluateitstransportabsorptioncharacteristicsandinvivobioavailabilityevaluationinrats．SnailenzymeconvertRbltoprepareCKwhichresearchresultsshowthattheenzymehydrolysismethodasakindofgreen，safemethod，andisabletokeeptheproductstructure，

3、moresuitableforindustrialproduction．Theoptimumenzymolysiswithsnailasewasachievedat50℃，pH5．5oftheaceticacid-sodiumacetate(1：1)buffersolutionfor36h．Thesubstrateconcentrationis1．0mg·mL_1．Withtheaboveconditions，thebioconversionrateis86．91％，andit’Sstructur

4、eWaselucidatedonthebasisofitsspectralevidences．Experiencing，optimizethepredictedresultsareingoodagreementwiththeexperimentalresults．Inaddition，theresearchgroupisalsostudyingtherareginsengsaponinothermonomers，inordertoprovidethematerialbasisforthestudy

5、ofrareginsengsaponincomponents，SOastomedicinerareactiveingredientsinnewproductdevelopmentprovidesaneffectivem。CaIlS．TheresultsofultrasoundassistedenzymaticpreparationofginsenosidesrareginsengsaponinCo·mpoundKshowsthattheuseofultrasound-assistedenzymat

6、icmethod，rareginsengsaponinCKconv—ersionrateincreasedfrom3．27％to6．91％．Themainreasonistheuseofultrasoniccavitation，vibr-ation，thermalandmechanicalaction，promptingthesubstratewiththeenzymeintotinyparticles，thecontactareaincreases，morefullycombinationfur

7、therfacilitatesplaySnailaseenzymatichydrolysis．MicrospheresimmobilizedenzymepreparationfindingssnailrareginsengsaponinCKshowthatt-hethermalstabilityoftheimmobilizedenzymesnail，pH，stabilityandenzymeimmobilizationsnail-shelflifehavebeeneffectivelyimprov

8、ed；atthesametime，snailenzymeimmobilizationstructurema—Yprotecttheenzymeandenzymaticchangesintheenvironmentwithoutchange；andtheimmobilizedenzymemaybeseparatedbyasimplemethodsuchasfiltrationorcentrifugation，andthenrecycling—g．Therefore，thepresen