vegf expression and cell apoptosis in nod mouse retina

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1、VEGFexpressionandcellapoptosisinNODmouseretina：CaiRuiLi,ShuGuangSun【摘要】Toinvestigateretinalvascularendothelialgroouseretina.METHODS:Animalsice)(2,4,6,8,12eLinkedImmunosorbentAssay)edtodetectVEGFlevelinbothserumandretina.Transmissionelectronmicroscopemethodineretinalcellapoptosis.RESULTS:par

2、edandretinasample,P<0.01;165.0±9.0μg/gversus18.0±4.0μg/ginretinalsample,P<0.01).ThereexistsapositivecorrelationbetVEGFandretinalVEGFlevelsintheearlydiabeticNODmice(γ=0.9902,P=0.001).ThenumberofthecellsapoptosisintheganglioncellsandendotheliumcanalsobeenfoundincreasedsignificantlyintheNODg

3、roup(P<0.01).CONCLUSION:ThehighVEGFexpressionmaybecontributedtoincreaseretinalcellsapoptosis.Manyfactorsassociatedightinvolveintheearlydiabetesstage.【关键词】retina;apoptosis;vascularendotheliumgroiceINTRODUCTION　　Diabeticretinopathyisthemostmondiabeticeyediseaseandaleadingcauseofblindness.Diabe

4、ticretinopathyischaracterizedincreasedmicrovascularpermeability,celldamage,abnormalbloodflo.Allthesefactorscancausecapillaryocclusion,microvasculardegenerationandabnormalneovascularization,theneventuallyleadingtoirreversibleblindness.Hyperglycemiaisanindependentriskfactorforthedevelopmentofc

5、ardiovasculardisease[1].Hoechanismsofhyperglycemiaandhyperglycemiaseffectontissuedamageandclinicalplicationsremainunclear.Vascularendothelialgroightplayaparticularlyimportantroleindiabeticretinopathy[2].TheexpressionchangesandeffectofVEGFonapoptosisinretinalcellsinducedbyhyperglycemiaarestille

6、lusive.　　Thenonobesediabetic(NOD)mouseisanusefulandimportantmodelofautoimmunetype1diabetes.TheincidenceofspontaneousdiabetesintheNODmousevariedfrom60%to80%infemalesand20%to30%inmales.Diabetesonsettypicallyoccursat12to14alemiceandslightlylaterinmalemice[3].ThisstudyaimstostudytheexpressionofVEGF

7、onapoptosisinretinalcellsindiabeticNODmice.　　MATERIALSANDMETHODS　　AnimalsNODmice(JacksonLaboratory,BarHarbor,ME)alhouseofCentralSouthUniversity.Miceent,continuouslyaccesstofoodandalexperimentsentfortheUseofAnimalsinOphthalmicandVi