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《芒柄花黄素对大鼠局部脑缺血再灌注损伤保护作用的初步研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ewe識状驅醮掘綱h乍用嗍御开究2017-12-1912:43:37中国医药导报2017年32期姜慈静++++++吴秋慧++++++任倩瑶++++++王W++++++陈健++++++卢慧玲［摘耍］鬥的探讨芒柄花黄紊对大鼠脑局部缺血再灌注损伤的神经保护作用。方法采用随机数字表法将42只SD雄性大鼠分为假手术组、缺血再灌注组和芒柄花黄素组，每组14只。芒柄花黄素组腹腔注射芒柄花黄素溶液20mg/kg，假手术组和缺血再灌注组注射等剂量生理盐水，连续7d。线栓法复制大鼠大脑屮动脉缺血再灌注模型，缺血时问1.5h。再灌注后24h各组分别进行神经功能缺损测定；干
2、/湿重法检测脑水肿程度；2,3,5-氯化三苯基四氮唑(TTC)染色计算脑梗死体积并检测血浆超氧化物歧化酶(SOD)活力、丙二醛(MDA)水平。结果与缺血再灌注组比较，芒柄花黄素组大鼠神经功能缺损评分降低，第二脑片梗死体积和第三脑片水肿程度减轻，血浆SOD活性捉髙，MDA水f•下降，差异均有统计学意义(P<0.05或P<0.01)。结论芒柄花黄素可改善大鼠脑缺血再灌注后祌经功能缺损程度，减小脑梗死体积，其机制可能与减低氧化应激水平有关。［关键词］芒柄花黄素；脑缺血再灌注损伤；植物雌激素；神经功能缺损［中图分类号］R743［文献标识码］A［文章编号］16
3、73-7210(2017)11(b)-0008-04［Abstract］ObjectiveToinvestigatetheprotectiveeffectofformononetinonlocalcerebralischemia-reperfusioninjuryinrats.MethodsAccordingtotherandomnumbertablemethod,42maleSDratsweredividedintoshamgroup，ischemia-reperfusiongroupandformononetingroup,with14ratsi
4、neachgroup.Theformononetingroupwasgivenformononetinsolution20mg/kgbyintraperitonealinjection,theshamgroupandischemia-reperfusiongroupwereinjectedwithsamedoseofnormalsaline,for7days.Suture-occludedmethodwasusedtocopythemodelofratswithcerebralischemia-reperfusioninjuryinmiddlecer
5、ebralartery,theischemictimewas1.5h.Afterreperfusionfor24h,theneurologicimpairmentofallgroupswasdetected；thedry/wetmethodwasusedtodetectthedegreeofcerebraledema；2，3,5-tripheyltetrazolium(TTC)stainingwasusedtocalculatethevolumeofcerebralinfarctionanddetecttheactivitiesofplasmasup
6、eroxidedismutase(SOD)andthelevelsofmalondialdehyde(MDA).ResultsComparedwithischemia-reperfusiongroup,thescoreofneurologicimpairmentinformononetingroupwasdecreased,thevolumeofcerebralinfarctionofthesecondbrainsliceandthedegreeofcerebraledemaofthethirdbrainslicewerereduced,theact
7、ivitiesofplasmaSODwereincreased,andthelevelsofMDAweredeclined,thedifferenceswereallstatisticallysignificant(P<0.05orP<0.01).ConclusionFormononetincanimprovethedegreeofneurologicimpairment,reducethevolumeofcerebralinfarction,themechanismmayberelatedtothedecreaseofoxidativestress
8、.［Keywords］Formononetin；Cerebralischemia-reperfusionin
