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《产朊假丝酵母、热带假丝酵母和酿酒酵母对活性紫kn-4r脱色的研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、cultutrecondition,C.tropicalisandS.cerevisiaewareselectedasthefermentationstrainsinthefollowingresearch.2.Theeffectsofdifferentconcentration（0.5%,1.0%,1.5%,2.0%）glucoseandammoniumsulfate,oxalicacid（1mM,3mMand5mM）anddifferentkindsofmetalirons(Cu2+,Mn2+,Na+andZn2+,theirconcentrati
2、onwereall5mM)ontheactivitiesofmanganeseperoxidase(MnP)andthede-colorizationratesofreactiveviolet4Rdye(100mg/L)ofC.tropicalisandS.cerevisiaewerestudied.Theresultsshowedthat:withtheprolongoftime,theactivitivesofMnPbothshowedincreasingtrendatfirstandgraduallyreduced,andachievedthem
3、aximumvalueonthefirstday.InbothC.tropicalisandS.cerevisiaefermentationsystems,comparedtothecontrol,0.5%glucoseincreasedsignificantlytheactivitiesofMnPrespectively,theactivitieswere1.20and1.79timesofthecontrol;but1.0%,1.5%and2.5%glucosedecreasedsignificantlytheactivitiesofMnP.0.5
4、%ammoniumsulfatecanincreasesignificantlytheproductionofMnP,theactivitieswerehigherthanthecontrol1.5U/L,1.8U/Lrespectively;and1.0%,1.5%and2.5%ammoniumsulfatehavesignificantinhibitoryeffects.Whenadding1mMoxalicacidtotheculturemedium,theproductionofMnPbyC.tropicalisandS.cerevisiaew
5、ereimproved,thehighestactivitiesofMnPwere6.2U/L,6.7U/Lrespectively,whichwerehighlythanthecontrol1.8U/L,2.2U/Lrespectively;but3mMand5mMofoxalicacidhavesignificantinhibitoryeffect.Cu2+,Mn2+andNa+allincreasedsignificantlyMnPactivities,andtheincreasedeffectsofCu2+werethebest,thehigh
6、estactivitiesofMnPwere5.8U/Land6.2U/Lrespectively,whichwereall1.32timesofthecontrol.InbothC.tropicalisandS.cerevisiaefermentationsystems,comparedtothecontrol,0.5%glucoseandammoniumsulfate,1mMoxalicacidincreasedsignificantlythede-colorizationratesofreactiveviolet4Rrespectively.Th
7、ede-colorizationratesofreactiveviolet4Rincreasedwiththeprolongoftime,theyachievedthehighestatthesixthday;but1.5%-2.0%glucoseandammoniumsulfate,5mMoxalicaciddecreasedsignificantlythede-colorizationratesofreactiveviolet4R.Whenadding0.5%glucosetobasicmediumofC.tropicalisandS.cerevi
8、siae,thede-colorizationratesofreactiveviolet4Rw
