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1、产朊假丝酵母论文：香蕉皮生物质发酵生产谷胱甘肽研究【中文摘要】谷胱甘肽（GSH）是由三种前体氨基酸形成的三肽化合物,作为一种抗氧化物广泛存在于生物细胞中,对于稳定胞内氧化【英文摘要】Glutathione,asatripetideofglutamate,cysteineandglycine,isoneofantioxidantsabundantinorganism.Itplaysanimportantroleinmaintainingthenormalcellularredoxenvironment.Soitiswid
2、elyusedinmedical,cosmetic,foodandhealthproducts.Lignocellulosicmaterials,suchasbananapeels,arelowpricerenewableresources.Itcutsthecostoffermentationusingthehydrolyzateofbananapeelsasthecarbonsource,whichalsobenefitstothereasonabletreatmentonbananapeels.Basedonth
3、efactthattheCandidautilishastheabilitytoutilizehexoseandpentosedegradedfromlignocellulosicbiomass,theGSHfermentationonthebananapeelsbyCandidautiliswascarriedoutinthisthesis.TheconditionsofGSHfermentationonxylosewereoptimizedbystatisticalmethodsandwedrewtheconclu
4、sionthatthefermentablesugarsinhydrolyzateofbananapeelswereanidealcarbonsource.Thentheproductionconditionsoffermentablesugarswereoptimizedbytheuniformdesignandthehighefficiencyfermentablesugarswereobtained.FurtherstudiesonthecellgrowthandGSHproductionbythediffere
5、ntdissolvedoxygenwerecarriedoutinthelastchapterofthisthesis.Themainconclusionswereasfollows:（1）Theanalysisonbananapeelsshowedthatitcontainedmassivecarbohydratesandlittlecrudeproteinandfat.Glucose,mannose,xyloseandarabinosewerefoundinthehydrolyzateofbananapeelsby
6、theHPLC.Basedonthesinglefactorexperiments,responsesurfacemethodologywasappliedforoptimizationtheGSHfermentationconditiononthexylose,andtheoptimalconditionswereasfollows:xylose20g/L,（NH4）2SO49.59g/L,KH2PO43g/L,L-methionine5.72g/L,MgSO40.20g/L,temperature30℃,volum
7、eofmedium50mL.TheGSHyieldontheoptimizedconditionswasimprovedby80.09%comparedtotheyieldonthestartingmedium.（2）Glucose,mannose,arabinoseandmixedsugars,werefurtherinvestigatedasthesolecarbonsourcebasedonthemediumcompositionsobtainedwithxylose.ItwasshownthatC.utilis
8、SZU07-01couldalsogrowwellonthemediumwiththesolecarbonsourceofglucoseandmannoseat20g/L.However,arabinosecannotbewellutilizedbytheyeastforGSHproduction,andthefermentati
