克班宁长循环脂质体的包封率、体外释放和稳定性研究

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1、翔翻銀flsteSS懷体飛獅口綻ffii开究2017-12-1912:34:39中国医药导报2017年31期刘娜+王琪蕾++陈凌云++马云淑rrAU•800•600-400-200-A■c0i■10i19(H间(mi")：克班宁对照品;B:克班宁长循坏脂质体样品;C:阴性对照品图1专属性考察色谱图表1克班宁脂质体溶液加样回收率试验绾果An人H(mg>測得量⑽m得a-梂品含aong)lnl收率<%)乎均阏收串(％)A1.76222.52171.679795.32101.561.76222.52051.678595.251.76222.53

2、291.690995.96屮0.8«ll1.71340.871498.91U8川1.70770.X65798.26O.HB1IL7I570.873799.16低0.17621.03360-1916108.780.17621.03950.1975112.150.1762I.O.i660.1946110.52表2克班宁包封率测定结J分离方法包封率（％）时长（1葡聚糖凝胶柱层析法79.464超滤法81.021［摘耍］0的研宄克班宁长循环脂质体的包封率、体外释放和稳定性。方法建立髙效液相色谱法测定长循环脂质体屮克班宁的含虽；分别采用葡聚糖凝胶柱层

3、析法和超滤离心法分离未包封克班宁，并对包封率和载药量进行测定；采用透析法考察克班宁长循环纳米脂质体的体外释放行为：考察不同温度和光照贮存条件下克班宁长循环脂质体外观、粒径和池漏率。结果采用葡萄糖凝胶柱层析法分离游离克班宁测得包封率为79.46%,采用超滤离心法测得包封率为81.02%，克班宁长循环脂质体载药量为5.6%:克班宁长循环脂质体体外释放规律拟合为Higuchi方程：y=0.1419xl/2+0.1475,R2值为0.9338;在40d闪克班宁长循环脂质体在避光4'C冷藏条件下贮存稳定。结论超滤离心法操作简单，重现性好，所制得的脂

4、质体具有明显的缓释释放特征，需避光冷藏贮存。［关键词］葡聚糖凝胶柱层析法;超滤离心法;包封率;载药fl;透析法［中图分类号］R284.1［文献标识码］A［文章编号】1673-7210(2017)11(a)-0004-05Studyonentrapmentefficiency,drugreleaseinvitroandstabilityofCrebaninelongcirculatingliposomeLIUNaWANGQileiCHENLingyunMAYunshuYunnanUniversityofTCM，YunnanProvince,

5、Kunming650500，China［Abstract］ObjectiveTostudytheentrapmentefficiency,drugreleaseinvitroandstabilityofCrebaninelongcirculatingliposome.MethodsTheconcentrationofCrebanineinlongcirculatingliposomewasdeterminedbyhighperformanceliquidchromatography.TheliposomeandfreeCrebaninew

6、ereseparatedbythesephadexgelcolumnchromatographyandthecentrifugalultrafiltration,theentrapmentefficiencyanddrugloadingweredetermined.Thereleasebehaviorofthedrugfromliposomewasstudiedbythedialysismethod.Theliposomeappearance,particlesizeandleakageratewerestudiedbytheeffect

7、sofdifferenttemperatureandlightstorage.ResultsTheentrapmentefficiencywas79.46%bythegelcolumnchromatographyand81.02%bythecentrifugalultrafiltration.Crebaninelong-circulatingliposomedrugloadingwas5.6%；theHiguchiequationofCrebaninelong-circulatingliposomeinvitroreleasewasy=0

8、.1419x1/2+0.1475，theR2valuewas0.9338；Crebaninelong-circulatingliposomewasstabilizedintheavoidlig