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1、)顧性B淋EM®白麵(B—ALL)调帝往T淋BO鹏做職肺份析2017-12-1912:35:09中国现代医生2017年31期张小薇++++++任建敏++++++傅丽敏++++++周灵玲［摘耍］鬥的分析兒童急性B淋巴细胞白血病(B-ALL)凋节性T淋巴细胞变化及调节机制。方法在2016年2月23円〜2017年2月23円期间选取50例健康体检者(对照组)、50例急性B淋巴细胞白血病患儿(观察组)为实验对象，对两组受检者均进行分离外周血CD4+T细胞、荧光定fiPCR(RT-PCR、REAL-TIMEPCR、总RNA定虽和提取、RT-PCR产物鉴定)、酶联免疫吸附试验、流式细胞
2、术检测，随后对比两组受检者各项检测结果。结果观察组患儿的CD28/CD4+Foxp3+(524.86±28.42)、ICOS/CD4+CD25highFoxp3+(725.69±15.74),TGF-pRII/CD4+(61.75±10.08)、Mtge-P(58.44±10.85)、IL-35p35(326.84±35.46)>IL-35EB13(128.56±12.74)、TGF-p(81.46±5.43)、IL-10(135.75±12.75)、ICOS+/CD4+CD25highFoxp3(3.86±1.75)%、Foxp3(812.43±56.32)、CD4+C
3、D25highFoxp3(9.85±2.45)%均商于对照组健康体检者的CD28/CD4+Foxp3+(158.33+21.06)ICOS/CD4+CD25highFoxp3+(352.12±10.03)、TGF-pRII/CD4+(28.42±5.89)、Mtge-p(23.17±5.33)、IL-35p35(154.28±26.31)、IL-35EB13(48.31±6.52)、TGF-p(29.51±6.38)、IL-10(24.91±5.22)、ICOS+/CD4+CD25highFoxp3(2.45±0.85)%、Foxp3(322.41±18.75)CD4+C
4、D25highFoxp3(4.12±1.75)%(P<0.05)。结论导致急性B淋巴细胞0血祸患儿出现亚群比例失调的主要原因为转化史长因子-pUransforminggrowthfactor-p，TGF-p)、分化群28(ClusterofDifferentiation28)>ICOS过度活化。［关键词］急性B淋巴细胞白血病；T淋巴细胞；凋节机制；酶联免疫吸附试验［中图分类号］R73［文献标识码］A［文章编号］1673-9701(2017)31-0005-04［Abstract］ObjectiveToanalyzethechangesandregulatorymechan
5、ismofregulatoryTlymphocytesinchildrenwithacuteBlymphocyticleukemia(B-ALL).MethodsAtotalof50childrenofhealthysubjects(controlgroup)and50childrenwithacuteBlymphocyticleukemia(observationgroup)whowereselectedfromFebruary23rd,2016toFebruary23rd,2017wereselectedasthestudysubjects.CD4+Tcellswer
6、eisolatedfromperipheralbloodinbothgroups,andquantitativePCR(RT-PCR,REAL-TIMEPCR,totalRNAquantificationandextraction,RT-PCRproductidentification),enzyme-linkedimmunosorbentassay,andflowcytometrywerecarriedout，andthentheresultsofeachtestwerecomparedbetweenthetwogroups.ResultsCD28/CD4+Foxp3+
7、(524.86±28.42)，ICOS/CD4+CD25highFoxp3+(725.69±15.74),TGF-pRII/CD4+(61.75±10.08),Mtge-P(58.44±10.85),IL-35p35(326.84±35.46),IL-35EB13(128.56±12.74),TGF-p(81.46±5.43),IL-10(135.75±12.75),ICOS+/CD4+CD25highFoxp3(3.86±1.75)%,Foxp3(812.43±56.32),CD4+CD25highFoxp3(9.85±2.
