家蚕foxo基因的克隆及其在丝腺程序性细胞死亡中的功能分析

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1、中文摘要，家蚕Foxo基因的克隆及其在丝腺程序性细胞死亡中的功能研究噬也可能发挥了抑制细胞凋亡或坏死，保护细胞的作用，这为家蚕细胞自噬与凋亡之间可能存在的联系提供了新的依据。关键词：家蚕；丝腺；FOXO；程序性细胞死亡；蜕皮激素；饥饿IV作者：周炳卿指导教师：徐世清协助指导教师：司马杨虎家蚕Foxo基因的克隆及其在丝腺程序性细胞死亡中的功能研究英文摘要CloningofFoxoGeneandtheFunctionalInvestigationinProgrammedCellDeathoftheBombyxmoriSilkGlandAbstractFOXOi

2、spivotalinphysiologicalprocessessuchasgrowth，development，CellDifferentiation，MetabolicApoptosis，SignalTransductionandSOforth．Inmammals，signaltransductionpathwaysofInsulin-likeGrowthFactorslikeIGF／P13K／FOXOarerelatedtoCellDivisionCycle，CellDeath，Stress，Detoxificationandsomeothercel

3、lprocesses·MetamorphosisfromlarvaetopupaeinBombyxmoriiscombinedwithfiercetissuerenewal，includingtherapiddegenerationandvanishingofspecifictissueslikesilkgland．Ithash’tbeenreportedwhether肋mbyxFOXOwasengagedinregulatingcelldifferentiationandapoptosisofspecific—statustissues．Pinpoint

4、ingtheProgrammedCell’Death：initiatedorinducedfromlarvaetopupae，theauthorclonedthecompletesequenceofFoxogeneandinvestigatedtheexpressionprofilesofit．Methodsofhormonetreatment，starvationandfoodrestrainwereconductedtoobservealterationsinFoxoexpressionlevelofsilkglandtissueinindividua

5、landBmNcellsandanyothergeneupstreamordownstreamthesignaltransductionpathways，aswellasvariationofFOXOproteinundercertaincircumstances，hopingtoprovidereferencetotheregulationmechanismofPCDgenesisduring肋mbyxmetamorphosis．Theresultsareasfollows：1．CloningandverificationofBombyxFoxogene

6、ThecompletemRNAsequenceofFoxogenewasclonedbyRACEandverifiedbytheobtainedFoxosegmentsinOurlaboratory．Thegenewas3326bp，andthelongeStORFwas1539bp．Itwaslocalizedtonscaf2890ofChromosome9with9extronsand8introns．Itencoded512aminoacids．Thetheoreticalmolecularweighofseducedproteinwas55679．

7、76wi血thefsoelectricpointof6．08．Twotrans-membranehelixesformilinertooutsideandonefromoutsidetoinnerwereobservedintheaminoacid402．434Varea．Thesecondarystructurepredictedthatc【helixofFOXOapproximatelytookby33．01％，extensionchainbyabout7．81％，13comerby3．21％andrandomrollingstructuretookb

8、yabout56．05％．Analysisoffunctional