视网膜色素上皮细胞诱导骨髓间充质干细胞分化为神经样细胞

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1、视网膜色素上皮细胞诱导骨髓间充质干细胞分化为神经样细胞：杨玲玲,周庆军,王瑶,王宜强【摘要】　　目的：检测视网膜色素上皮细胞对骨髓间充质干细胞分化的调节作用。方法：体外培养人骨髓间充质干细胞（BMSC）和视网膜色素上皮（RPE）细胞。RPE细胞采用紫外线照射处理，而后与CFSE标记的BMSC共培养14d。并在共培养体系中加入牛眼视网膜提取物（BRE）以研究视网膜成分对此分化过程的影响。采用NSE,Nestin和GFAP抗体标记检测BMSC分化前后的表达特征。结果：BMSC在与RPE细胞共培养后，能够分化成为神经样细胞，并表达神经性细胞的特异性标记NSE，Nestin和GFAP

2、。BRE能够显著促进共培养体系中BMSC向神经样细胞的分化。结论：RPE和BRE能够诱导BMSC分化为神经样细胞。【关键词】髓间充质干细胞；视网膜色素上皮细胞；神经样细胞；牛眼视网膜提取物　　AbstractAIM:Todetectthedifferentiationeffectsofretinalcellsorextractsonbonemarroesenchymalstemcells(BMSC).METHODS:HumanfetalBMSCidylester(CFSE),andcoculturedentepithelial（RPE）cellseassays,aret

3、inalextractofbovineretinalextract(BRE)munostainingedtodeterminethecharacteristicsofBMSC.RESULTS:TheresultsindicatedthatbycoculturedarkersNestin,GFAPandNSE.Andtheexpressionofthesemarkersarroesenchymalstemcells;retinalpigmentepithelialcells;neurallikecells;bovineretinalextract　　INTRODUCTIO

4、N　　Accordingtotheoriginandpotentialofdifferentiation,tcellscanbedistinguished:embryonicstemcells(ESC)andsomaticstemcells(SSC).SSCareisolatedfromfetal(aftergastrulation)oradulttissues,butclassicallythedifferentiationfateofthesecellsarebelievedtobelimitedtothecelltypesthatbelongtothetissuefro

5、mcellsmightbeabletodifferentiateintocelltypesofotherlineages[1].Bonemarroesenchymalstemcells(BMSC)areoneofthemajorsubpopulationsofSSC,icalsinculturemedium.Theagentsincludeβmercaptoethanolanddimethylsulfoxide,epidermalgroethylxanthine/dibutyrylcAMP,or5AzaC/groallproportionofBMSCderivedce

6、llsdifferentiatedintoneuronlikecellsexpressingNeuNandglialcellsexpressingGlialfibrillaryacidicprotein(GFAP)esencephalicorstriatalcells.Theretinalpigmentepithelium(RPE)isahexagonallypackedmonolayercellinocularretinathatperformscriticalfunctionsinthemaintenanceofthephysiologyofphotoreceptors

7、theouterlayeroftheopticcup.TheresearchofChiouetal[8]shoanBMSCanretinalpigmentepithelium(HRPE)cellsfacilitatestheretinaandphotoreceptorlineagedifferentiationofadulthumanBMSC.ThissystemindicatestheretinaldifferentiationpotentialofBMSC.Inthisstudy,bydirec