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1、X线照射对实验性癫痫大鼠脑皮层GABA和GABAA受体的影响作者：陈家玉，梁树立，曾啸雄【摘要】目的探讨癫痫大鼠放射治疗中丫-氨基丁酸（GABA）及其受体的作用机制并选择合理放射剂量。方法利用慢性点燃癫痫大鼠对照组、放射1组、放射3组进行0、24、6Gy的X线放射，对放射2组、放射4组和放射5组行12Gy的X线放射，每组10只，对照组和放射1组、放射3组于放射后lh断头取脑，放射4组于放射后1天、放射5组于放射后1周进行断头取脑，并观察放射5组其放射后癫痫发作情况，用氨基酸分析仪测定各组癫痫大贏额叶皮层内的GABA
2、含量变化，利用免疫组织化学方法观察抗GABAA受体阳性率。结果放射5组于照射后1周内癫痫在鼠未出现诱发癫痫发作。放射1组的GABA含量为（士）ng，GABAA受体阳性率为（士）％，均明显高于对照组，12Gy照射后lh、24h、1周后均较对照组间GABA含量高，其中以24h最为明显，分别为（土）ng，（士）ng和（土）ng。抗GABAA受体阳性率在照射后1周内基本稳定于较高水平。结论放射治疗癫痫主要是通过发生快速而持续的GABA与GABAA受体变化发生作用，癫痫大鼠接受12Gy的放射治疗较为合理。【关键词】Y氨基丁酸
3、受体GABA-A癫痫大鼠X线Abstract:ObjectiveToinvestigatetheroleofGABAandGABAAreceptorplayinradiotherapyofepilepticrat,andtolookfortheappropriatedoseofradiotherapyforepilepsy.MethodsAchronicpentylenetetrazolechemicalkindlingratmodelofepilepsywasreproduced,andtheratsweredi
4、videdforcontrolgroupandradiogroupslto5withlOratsineachgroup.Controlgroup,groupsland3weregivenO,24,or6GyXrayirradiationtothebraininturns,andgroups2,4and5weregivenl2GyXrayirradiation,theratsincontrolgroup,groups1,2,and3werekilledlhour,andgro叩4waskilledondayl,and
5、group5waskilledinlweekafterradiotherapy.ContentofGABAwasmeasuredbyaminoacidanalyzer,andGABAAreceptorchangeswereassessedbyimmunohistochemicalstaininginfrontallobeofthebrainofratsinallgroups.Seizureofratingroup5wererecordedinlhour,24hoursorlweekafterirradiation.
6、ResultsTheepilepticratsingroup5didn，tsufferfromseizureafterradiotherapy.Ingroupi,thequantityofGABAwas（土）ng，andpositiverateofGABAAreceptorwas（土）%,whichwerehigherthanthoseincontrolgroup,theeontentofGABAinratsreceivedl2GyX-rayradiotherapywere(土)ng，(士）ngand（士）ngin
7、lhour,Idayandlweekafterirradiation,andsignificanthigherthanthatincontrolgroup,also,thepositiverateofGABAARinthoseratsweresignificanthigherthanthatincontrolgroup.ConclusionsTheefficacyofradiotherapyforepilepticratmightbeattributabletorapidandsustainedchangeinGA
8、BAandGABAAreceptors,andl2Gyappearedtobetheappropriateirradiationdoseforepilepticrat.Keywords:GABA;receptor,GABA-A:epilepsy;rats;X-rays临床发现放射治疗对癫痫发作可能有抑制作用，但其作用机制尚不明确。在癫痫的发作与治疗机制中Y氨
