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1、X线照射对实验性癫痫大鼠脑皮层GABA和GABAA受体的影响作者：陈家玉，梁树立，曾啸雄【摘要】 目的探讨癫痫大鼠放射治疗中γ-氨基丁酸(GABA)及其受体的作用机制并选择合理放射剂量。方法利用慢性点燃癫痫大鼠对照组、放射1组、放射3组进行0、24、6Gy的X线放射，对放射2组、放射4组和放射5组行12Gy的X线放射，每组10只，对照组和放射1组、放射3组于放射后1h断头取脑，放射4组于放射后1天、放射5组于放射后1周进行断头取脑，并观察放射5组其放射后癫痫发作情况，用氨基酸分析仪测定各组癫痫大鼠额叶皮层内的GABA含量变化，利用免疫组织化学方法
2、观察抗GABAA受体阳性率。结果放射5组于照射后1周内癫痫在鼠未出现诱发癫痫发作。放射1组的GABA含量为(254.16±44.68)ng，GABAA受体阳性率为(39.56±7.22)%，均明显高于对照组，12Gy照射后1h、24h、1周后均较对照组间GABA含量高，其中以24h最为明显，分别为(252.09±33.89)ng，(348.73±56.00)ng和(258.02±62.95)ng。抗GABAA受体阳性率在照射后1周内基本稳定于较高水平。结论放射治疗癫痫主要是通过发生快速而持续的GABA与GABAA受体变化发生作用，癫痫大鼠接受12G
3、y的放射治疗较为合理。【关键词】γ氨基丁酸受体GABA-A癫痫大鼠X线 Abstract:ObjectiveToinvestigatetheroleofGABAandGABAAreceptorplayinradiotherapyofepilepticrat,andtolookfortheappropriatedoseofradiotherapyforepilepsy.MethodsAchronicpentylenetetrazolechemicalkindlingratmodelofepilepsywasreproduced,andtherats
4、weredividedforcontrolgroupandradiogroups1to5with10ratsineachgroup.Controlgroup,groups1and3weregiven0,24,or6GyXrayirradiationtothebraininturns,andgroups2,4and5weregiven12GyXrayirradiation,theratsincontrolgroup,groups1,2,and3werekilled1hour,andgroup4waskilledonday1,andgroup5wask
5、illedin1weekafterradiotherapy.ContentofGABAwasmeasuredbyaminoacidanalyzer,andGABAAreceptorchangeswereassessedbyimmunohistochemicalstaininginfrontallobeofthebrainofratsinallgroups.Seizureofratingroup5wererecordedin1hour,24hoursor1weekafterirradiation.ResultsTheepilepticratsingr
6、oup5didn'tsufferfromseizureafterradiotherapy.Ingroup1,thequantityofGABAwas(254.16±44.68)ng，andpositiverateofGABAAreceptorwas(39.56±7.22)%,whichwerehigherthanthoseincontrolgroup,thecontentofGABAinratsreceived12GyX-rayradiotherapywere(252.09±33.89)ng,(348.73±56.00)ngand(258.02±6
7、2.95)ngin1hour,1dayand1weekafterirradiation,andsignificanthigherthanthatincontrolgroup,also,thepositiverateofGABAAR6inthoseratsweresignificanthigherthanthatincontrolgroup.ConclusionsTheefficacyofradiotherapyforepilepticratmightbeattributabletorapidandsustainedchangeinGABAandGA
8、BAAreceptors,and12Gyappearedtobetheappropriateirradiationdose
