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《bcl-2基因与神经生长因子（ngf）抑制神经细胞凋亡的研究》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、Bcl-2基因与神经生长因子(NGF)抑制神经细胞凋亡的研宄韩贵和魏威顾军(浙江中医药大学附属中丙医结合医院骨科浙江杭州310003)【摘要】目的探讨原癌基因bcl-2与祌经生长因子(NGF)联合应用对抑制祌经细胞凋亡的协同作用。方法培养PC12细胞至对数生长期，用100感染复数(multiplicityofinfection,MOI)的携带bcl-2基因的慢病毒质粒及未携带bcl-2基因的慢病毒质粒感染PC12细胞。再将其分为A、B、C、D、E、F六组，A组为bcl-2-PC12细胞培养液中不加H2O2及NGF(bcl
2、-2-PC12组)；B组为bcl-2-PC12细胞培养液中加H2O2(bcl-2-PC12+H2O2组)；C组为bcl-2-PC12细胞培养液中加H2O2及NGF(bcl-2-PC12+H2O2+NGF组)。D组为NC-PC12细胞培养液中不加H2O2及NGF(NC-PC12组);E组为NC-PC12细胞培养液中加H2O2(NC-PC12+H2O2组)；F组为NC-PC12细胞培养液中加H2O2及NGF(NC-PC12+H2O2+NGF组)。皮用流式细胞仪检测各组细胞的凋亡率，釆用BCA(bicinchoninicaci
3、d)法检测bcl-2基因表达蛋白浓度，数据进行统计学分析。结果A组细胞凋亡率较D组低(u=2.16,0.02<P<0.05),B组较E组低(u=2.38，0.01<P<0.02)，C组较B组低(u=2.27,0.02<P<0.05)及较F组低(u=2.83,0.02<P<0.05),统计学分析有显著性差异。A组bcl-2基因表达蛋白浓度高于D组(t=2.87,0.005<P<0.002),B组高于E组(t=2.75,0.01<P<0.05),C组高于
4、B组(t=2.16,0.02<P<0.05)及F组(t=2.23,0.02<P<0.05)，统计学分析有显著性差异。结论bcl-2基因及NGF均对正常神经细胞的凋亡有抑制作用，能够增强神经细胞的抗损伤能力，二者联合应用时具有协同作用。【关键词】bcl-2基因祌经生长因子祌经细胞凋亡【中图分类号】R39【文献标识码】A【文章编号】2095-1752(2013)27-0056-03Abaissementroleofbcl-2geneandNGFontheapoptosisofnervecellsHAN
5、Guihe,WEIWei,GUJunl(Departmentoforthopaedics,TheAffiliatedClinicalHospitalofIntegratedTraditionalandWesternMedicineofZheJiangUniversityofTraditionalChineseMedicine,ZhejiangHangzhou,310003,China)【Abstract】ObjectiveToapproachsynergiaabaissementroleoftheproto-oncoge
6、nebcl-2andNGFontheapoptosisofnervecells.MethodsPC12cellswereculturedtothelogarithmicgrowthphase,slowvirusplasmidcarryingthebcl-2geneandslowvirusplasmidinfectedrespectivelyPC12cellsby100MOl.Thentheyweredividedintosixgroups(groupA,groupB,groupC,groupD,groupEandgrou
7、pF).GroupA:PC12cellswithslowvirusplasmidcarryingthebcl-2gene.GroupB:PC12cellswithslowvirusplasmidcarryingthebcl-2gene,whichweredamagedbyH2O2.GroupC:PC12cellswithslowvirusplasmidcarryingthebcl-2gene,whichweredamagedbyH2O2,weretreatedwithNGF.GroupD:PC12cellswithslo
8、wvirusplasmid.GroupE:PC12cellswithslowvirusplasmid,whichweredamagedbyH2O2.GroupF:PC12cellswithslowvirusplasmid,whichweredamagedbyH2O2.,weretreatedwithNGF.Thera
