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1、CellBiologyExperiments1.ObservationofCellularMorphologyandStructure(4periods)2.CellFusion-PEGInduced(4periods)3.PreservationandRecoveryofBacteriaStrains(4periods)4.Cellproliferationandaberrationofchromosome(4periods)5.ExtractionofNucleicAcids(4periods)6.DisplayandObservationofplantcytoskeleton(4p
2、eriods)7.Planttissueculture(6periods)LaboratoryRules1.Alwayswearawhitelaboratoryjacketwhenyoudopracticalwork穿实验服2.Youarerequestedtocomeontime,andnotallowedtoleaveearly不迟到早退3.Anyuseoftheinstrumentsmustberegisteredorrecorded实验仪器登记4.Cleanthelaboratoryafteryouhavefinishedapracticalinvestigation打扫卫生5.
3、Toxicmaterialsshouldbehandledunderyourinstructor’sdirection有毒物品的处理ObservationofCellularMorphologyandStructure1.Objectives目的2.Background背景知识/原理3.Materials实验材料4.Procedure实验程序5.ResultsandReport结果和报告ExperimentOne1.Objectives1.1Learnmoreabouttheuseoflightmicroscope学习使用光学显微镜1.2Befamiliarwiththebasicmor
4、phologyandstructureofanimalandplantcells熟悉动物和植物细胞的基本形态和结构1.3Masterthemethodofdrawingimagesunderlightmicroscope掌握在光学显微镜下画图的方法2.BackgroundCellisthebasicunitofbiology,nomatterinthemorphologyorintheirfunction.细胞是生物的基本单位，无论形态、功能Therearemanykindsofcellswithdifferentmorphologies,whichisgenerallyassociat
5、edwiththeirfunctions.各种各样的形态，通常与功能相适应Redbloodcellsshowabiconcave（双面凹陷）shapeinthesurface,whichisconvenientforitsflowinbloodvessel.Nervecellscansensestimulationbysomeunmatchedbranchingoutgrowth（长短不一的分支）.Whilemacrophagesshowirregularedges,whocanprojectsomepseudopod（伪足）toswallowpathogens(病菌).Thecells
6、’volumevariesgreatly.Someofthecellscanbeobservedbyournakedeyes,形态变化多样，有的肉眼可见suchasthebird’seggs,butmostofthemareinvisiblewithoutusingamicroscope.例如鸟蛋Ananimalcelliscomposedofcellmembrane,nucleus,cytoplasm,andsomeorganelles,suchasendoplasmicreticulum,Golgibodyandsoon.动物细胞Unlikeanimalcells,plantcell
7、saresurroundedbyathick,rigidcellwall.植物细胞3.Materials3.1Binocularmicroscope,microscopeslides,coverslip,双目显微镜、载玻片、盖玻片3.2Oralepidermalcells口腔上皮细胞3.3Onionbulb,tomato洋葱鳞茎，番茄3.41%iodinestain（碘液）,1%toluidineblue（甲苯胺蓝）,toothpi
