化学缺氧以及外源性血管内皮生长因子对脐静脉内皮细胞可溶性血管内皮生长因子受体-mrna表达影响的研究论文

《化学缺氧以及外源性血管内皮生长因子对脐静脉内皮细胞可溶性血管内皮生长因子受体-mrna表达影响的研究论文》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、华中科技大学硕士学位论文用呈现剂量依赖关系；3.VEGF在拮抗CoCl2诱导脐静脉内皮细胞缺氧损伤时，细胞内sFlt-1mRNA表达强度下降，其作用呈剂量依赖关系。关键词：妊娠高血压疾病；人脐静脉内皮细胞；可溶性血管内皮生长因子受体-1；血管内皮生长因子3华中科技大学硕士学位论文StudyontheeffectsofchemicalhypoxiainducedbycobaltchlorideandextraneousVEGFoncellinjury,proliferationandtheexpressionofsolub

2、levascularendothelialgrowthfactorreceptor-1mRNAofhumanumbilicalveinendothelialcells.AbstractPart1Chemicalhypoxiaeffectsofcobaltchlorideoncellinjury,proliferationandtheexpressionofsolublevascularendothelialgrowthfactorreceptor-1mRNAofhumanumbilicalveinendothelia

3、lcells.Objective:investigatethechemicalhypoxiaeffectofcobaltchlorideonthecellinjury,proliferation,andtheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1)ofhumanumbilicalveinendothelialcells.Methods:cellinjurywasevaluatedbycellviability(MT

4、Tassay),lacticdehydrogenase(LDH)releasedfromallgroupHUVECsintothemediumweremeasuredbyusingLDHkits,andtheexpressionofsFlt-1ofallgroupwasdetectedbyusingthequantitativereversetranscriptase-polymerasechainreaction(RT-PCR).Results:cobaltchloridecouldinjuryHUVECs,induc

5、edtheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1),andtherewasatimedependentmanner.Conclusion:HUVECswereinjuredinducedbyCOCl2,proliferativeabilitywaslessenedandtheexpressionofsFlt-1mRNAwasup-regulated,theexpressionofsFlt-1mRNAofHUVECs

6、mayplayanimportantroleinthedevelopmentofhypertensivedisordercomplicatingpregnancy.Keywords:Hypertensivedisorderscomplicatingpregnancy;Humanumbilicalveinendothelialcell;solublefms-likekinasreceptor-1;chemicalHypoxia;4华中科技大学硕士学位论文Part2Theinvestigationontheagonistic

7、effectofextraneousVEGFontheinjuredHumanumbilicalveinendothelialcellsinducedbyCoCl2Objective:toinvestigatetheeffectofextraneousVEGFontheexpressionofmRNAofsolublevascularendothelialgrowthfactorreceptor-1(sFlt-1)ofhumanumbilicalveinendothelialcellsinducedbychemicalh

8、ypoxiawithCoCl2Methods：Thecellshadbeenculturedunderserumstarvationconditionfor12hour,whichwererandomlydividedintothefollowinggroups:①normalcontrolgroup(cellswe