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《黄芪对高糖环境下人脐静脉内皮细胞增殖及血管内皮细胞生长因子表达的影响研究》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、黄芪对高糖环境下人脐静脉内皮细胞增殖及血管内皮细胞生长因子表达的影响研究?1862?PJCCPVDNovember2Ol1.V01.19No.1l黄芪对高糖环境下人脐静脉内皮细胞增殖及血管内皮细胞生长因子表达的影响研究吴银侠,宋少武,彭军?论着?【摘要】目的探讨黄芪对人脐静脉内皮细胞(VEC一304)增殖及血管内皮细胞生长因子(VEGF)表达的影响.方法以不同浓度的葡萄糖,黄芪,不同浓度黄芪联合24hlC50葡萄糖作用于VEC一30424h,观察细胞生长状态变化.MTr法检测细胞生长率.应用RT—PCR检测24hlC50葡萄糖组,黄芪联合24hlC50葡萄糖组VE
2、GF的mRNA表达;Westernblot检测葡萄糖组,黄芪组和黄芪联合24hlC50葡萄糖组VEGF蛋白水平.结果(1)不同浓度的葡萄糖和低浓度黄芪(50mg/L)联合24hiC50葡萄糖对细胞起抑制作用,可显着抑制VEC一304的生长;高浓度黄芪及高浓度黄芪(~300mg/L)联合24hlC50葡萄糖对细胞有增殖作用.(2)葡萄糖组VEGFmRNA和蛋白表达下调,高浓度黄芪联合24hlC50葡萄糖组VEGFmRNA和蛋白表达正常.结论高浓度葡萄糖抑制VEC一304增值,高浓度黄芪促进VEC一304增值,黄芪可保护高浓度葡萄糖所致的血管损伤.【关键词】黄芪;葡萄
3、糖;血管内皮;增殖;抑制【中图分类号】R282【文献标识码】A【文章编号】1008—5971(2011)11—1862—03ExperimentalStudyOI1theProtectiveEffectsofRadixAstragaliOilVEC一304ExposedtoHighLevelofGluoseWUYin—xia,SONGShao一u,PENGJun.ClinicalCollege,WuhanUniversityofScienceandTechnology,Wuhan430064,China【Abstract】ObjectiveToinvestigat
4、etheofprotectiveeffectsofradixastragalionthemultiplicationoftraumaticvascu—larendothelialcellsandtheexpressionofvascularendothelialgrowthfactorexposed.MethodsVascularendothelialcells,VEC一304weretreatedwithvariousconcentrationsofglucose,radixastragaliandthecombinationofgluoseandradixas
5、tragali.Mor—phologicalchangesofVEC一304wereobservedin3groupsafter24hinvitro.ThegrowthratiowasmeasuredbyMTrassay.TheexpressionofVEGFmRNAinVEC一304wastestedbyRT—PCRintwogroups(24hlC50glucosegroup,radixastragaligroupcombinedwith24hlC50glucose).TheexpressionofproteinVEGFinthreegroups(glucos
6、egroup,radixastragaligroupandthecombinationgroupofradixastragaliand24hlC50glucose)wereexaminedbyWesternblotrespectively.Results(1)thereweresignificantinhibitoryeffectsonTheVEC—-304treatedwithdifferentconcentrationsglucoseandthecombinationoflightcon..eentrationradixastragaliand24hlC50S
7、luose.comp~edwithcontrolgroup,thegrowthofVEC一304couldbepromotedbythehighconcentrationradixastragaliandthecombinationofhighconcentrationradixastragaliand24hlC50gluose.(2)Theexpres?sionofVEGFmRNAandproteinweredepressedinglucosegroup,butitWasatnormallevelingroupofhighconcentrationradixas
8、traga
