卡立泊来德对离体肺缺血再灌注损伤的初步实验研究.doc

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1、卡立泊来德对离体肺缺血再灌注损伤的初步实验研究作者：周荣华，陈果，蒲江涛，陈艳芳，刘馨烛，张艳菊，龙村，刘斌【摘要】目的研究选择性钠/氢离子(Na+/H+)交换阻断剂卡立泊来德(Cariporide)对离体大鼠肺缺血/再灌注损伤的防治效果。方法建立离体大鼠肺缺血再灌注损伤模型。18只SD成年大鼠随机分为3组，每组6只：对照组:采用含5％羟乙基淀粉的KHHB(Krebs-Henseleithydroxyethylamylopectinebuffer)灌注液持续平衡灌注、通气120min；缺血再灌注组:平衡

2、30min后，缺血60min、再灌注30min；Cariporide组:平衡15min，给予Cariporide预处理15min，缺血60min、再灌注30min。持续监测并记录肺动脉压(PAP)；留取再灌注末灌注液,测定超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量；再灌注结束，测肺湿/干重比(W/D)；光、电镜观察肺病理学改变。结果缺血再灌注组PAP、MDA含量、SOD活性和W/D较对照组明显升高(P<0.05)，SOD活性显著下降(P<0.05)。Cariporide能明显减少PA

3、P、MDA和W/D的增加，提高SOD活性(P<0.05)，显微、超微结构证实Cariporide组肺血管内皮及肺泡上皮的病理变化明显减轻。结论Cariporide能降低离体大鼠肺缺血再灌注损伤。　【关键词】Na+/H+交换；卡立伯来德；肺；缺血再灌注损伤　　Abstract:OBJECTIVEToinvestigatetheprotectiveeffectsofselectiveNa+/H+exchangercariporideonisolatedratlungischemiaandreperfu

4、sioninjury.METHODSTheisolatedlungischemia/reperfusionmodelswereestablished.Afterequilibration,eighteenadultS.Dratswererandomlydividedintothreegroups:thecontrolgroup(n=6),theischemia/reperfusiongroup(n=6)andthecariporidegroup(n=6).Inthecontrolgroup,theiso

5、latedratlungswerecontinuouslyperfusedwiththeKrebs-Henseleithydroxyethylamylopectinebuffer(KHHB)solutionfor120min.Intheischemia/reperfusiongroup,theisolatedratlungswerereperfusedfor30minafter60minofischemia．Inthecariporidegroup,cariporidewasadministeredin

6、theKHHBsolutionbeforeischemia.Pulmonaryarterypressure(PAP)werecontinuouslymonitored.TheKHHBsolutionat30minofrepefusionswascollectedtomeasurethemalondialdehyde(MDA)contentandsuperoxidedismutase(SOD)activity.Afterreperfusion,wettodrylungweightratio(W/D)was

7、measured.Themorphologicchangeswerealsoobservedunderlightandelectronicmicroscopes.RESULTSPAP,MDAandW/Dintheischemia/reperfusiongroupweresignificantlyhigherthanthoseinthecontrolgroup(P<0.05),andtheSODactivitywaslowerthanthatin6thecontrolgroup(P<0.05)

8、.While,comparedwiththeischemia/reperfusiongroup,cariporidesignificantlyreducedthePAP,MDAandW/D(P<0.05),andsignificantlyincreasedtheSODactivity(P<0.05).Themorphologicandultrastructuraldamagesinthecariporidegroupweremi