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1、JournalofHistochemistry&Cytochemistryhttp://jhc.sagepub.com/Localizationofhyaluronicacidinhumanarticularcartilage.AAsari,SMiyauchi,SKuriyama,AMachida,KKohnoandYUchiyamaJHistochemCytochem199442:513DOI:10.1177/42.4.8126377Theonlineversionofthisarticlecanbe
2、foundat:http://jhc.sagepub.com/content/42/4/513Publishedby:http://www.sagepublications.comOnbehalfof:OfficialJournalofTheHistochemicalSocietyAdditionalservicesandinformationforJournalofHistochemistry&Cytochemistrycanbefoundat:EmailAlerts:http://jhc.sagep
3、ub.com/cgi/alertsSubscriptions:http://jhc.sagepub.com/subscriptionsReprints:http://www.sagepub.com/journalsReprints.navPermissions:http://www.sagepub.com/journalsPermissions.nav>>VersionofRecord-Apr1,1994WhatisThis?Downloadedfromjhc.sagepub.combyguestonO
4、ctober11,20110022-1554/94/$3.30TheJoumalofHistochemistryandCytochemistryVol.42,No.4,pp.113-522,1994Copyright01994byTheHistochemicalSociety,Inc.PrintedinU5.A.OriginalArticle1LocalizationofHyaluronicAcidinHumanArticularCartilageAKIRAASARI,SATOSHIMIYAUCHI,S
5、ETSUROKURIYAMA,AKEMIMACHIDA,KUNIOKOHNO,andYASUOUCHIYAMATokyoResearchInstitute(AA,SM,AM),SeikagakuCorporation,Tokyo,Japan;DepartmentofOrthopedicSurgery(SK),NipponKohkanHospital,Kzwasaki,Japan;DepartmentofAnatomy(KK),InstituteofBasicMedicalSciences,Univers
6、ityofTsukuba,Tsukuba,Ibaraki-ken,Japan;andtheDepartmentofCellBiologyandNeuroanatomyVU),lwateMedicalUniversitySchool'ofMedicine,Morioka,Iwate-ken,Japan.ReceivedforpublicationJuly19,1993andinrevisedformNovember2,1993;acceptedNovember6,1993(3A3089).Todemons
7、tratelocalizationofhyaluronicacid(HA)inar-oftrypsinwithCHaseABCabolishedintra-andextracellu-ticularcartilageofthehumanfemur,biotinylatedHA-larstainingforHAinallzones.Bymicrobiochemicalstudy,bindingregion,whichspecificallybindsHAmolecules,wastheconcentrat
8、ionsofHAanddermatansulfatewerehighappliedtothetissue.Insectionsftedby2%paraformalde-inthemiddlezone,whereasthoseofchondroitinsulfateandhyde-2%glutaraldehyde,HAstainingwasdetectedinlam-keratansulfatewerehighinthedeepzone.Th
