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1、Rheumatology2007;46:1769–1772doi:10.1093/rheumatology/kem234AdvanceAccesspublication17October2007ConciseReportPlatelet-releasedgrowthfactorsenhancethesecretionofhyaluronicacidandinducehepatocytegrowthfactorproductionbysynovialfibroblastsfromarthriticpatients1231121E.Anitua,M.Sa
2、´nchez,A.T.Nurden,M.M.Zalduendo,M.delaFuente,J.AzofraandI.Andı´aObjectives.Autologousplatelet-secretedgrowthfactors(GFs)mayhavetherapeuticeffectsinosteoarthritis(OA)capsularjointsviamultiplemechanisms.Ouraimwastoexaminetheeffectofaplatelet-derivedpreparationrichingrowthfactors(
3、PRGFs)inOAsynovialcellbiology.Methods.Synovialcellswereisolatedfrom10osteoarthriticpatientsandculturedinserum-freemedia(basalconditions)andexposedtoeitheraplatelet-poorpreparationorPRGFfor72h.Cellsactivatedwithinterleukin-1(IL-1)for48hwerealsoexposedtoPRGF.Changesinseveralevent
4、srelevanttojointhomeostasisincluding(i)hyaluronicacid(HA)secretion,(ii)thebalancebetweenmetalloproteinase-1,-3and-13(MMP-1,MMP-3andMMP-13)andtissueinhibitor-1(TIMP-1)and(iii)thesecretionoftransforminggrowthfactor-1(TGF-1),vascularendothelialgrowthfactor(VEGF)andhepatocytegrowth
5、factor(HGF),wereallassessed.Results.PRGFsignificantlyenhancedHAsecretioncomparedwithplatelet-poorpreparations,P<0.05;atthesametimereleaseofTIMP-1,MMP-1,MMP-3andMMP-13werenotaffected.AnincreasedHGFproductionwasobserved(P<0.05)butVEGFandTGF-1levelsremainedunchanged.PRGFsignifican
6、tlyenhancedthesecretionofHAinducedbyIL-1activation,P<0.05,butitdidnotmodifytheDownloadedfromIL-1-inducedriseinMMP-1,MMP-3andVEGF.Incontrast,PRGF-inducedHGFproductionwasabolishedbythepresenceofIL-1duringPRGFtreatment,P<0.05.Conclusions.Intra-articularadministrationofPRGFmightbeb
7、eneficialinrestoringHAconcentrationandswitchingangiogenesistoamorerheumatology.oxfordjournals.orgbalancedstatusbutdoesnothalttheeffectsofIL-1onsynovialcells.KEYWORDS:Platelet-richplasma,Osteoarthritis,Synovialcells,IL-,Growthfactors.IntroductionchondrocyteswhileincreasesinTIMP-
8、1intheextracellularspacemayacceleratecomplexformationw
