透明质酸correlated metabolismo f proteoglycans and hyaluronic acidin

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1、THEJOURNALOFBIOLOGICALCHEMISTRYVol.263,No.8,IssueofMarch15,pp.3632-3638,1988PrintedinU.S.A.CorrelatedMetabolismofProteoglycansandHyaluronicAcidinBovineCartilageOrganCultures*(Receivedforpublication,August24,1987)TeresaI.MoralesandVincentC.HascallFromtheBoneResear

2、chBranch,NationalInstituteofDentalResearch,NationalInstitutesofHealth,Bethesda,Maryhnd20892ProteoglycansexistincartilageascomplexesinThepioneeringstudiesofFellandJubb(5)showedthatwhichmanyproteoglycanmoleculesareboundtoaproteoglycanresorptionincartilageisdirectly

3、underthecentralfilamentofhyaluronicacid.Manystudieshavecontrolofresidentchondrocytes.Sandyetal.(6)subsequentlyinvestigatedchangestakingplaceinproteoglycanshowedthatproteoglycansreleasedfromculturesofrabbitmonomerstructureduringcartilagecatabolismusuallyarticularc

4、artilageduringthecatabolicprocesshadlargehy-undertheassumptionthathyaluronicacidisarela-tivelyinertmetaboliccomponentofthecomplex.Indrodynamicsizesbutwereunabletobindtohyaluronicacid.thispaperwepresentorganculturedatasupportingaTheyproposedthatthemechanismofrelea

5、seinvolvedanewhypothesisthatthecatabolismofproteoglycanshydrolyticcleavageabovethehyaluronicacidbindingregionandhyaluronicacidarecoordinatelyregulatedbyoftheproteoglycancoreprotein.Laterstudiesbyothergroupschondrocytes.Thedataindicatesthat:1)newlysyn-havebeencons

6、istentwiththishypothesis(7-9),althoughthethesizedhyaluronateandproteoglycanmaintainaissuehasnotbeendefinitelysettled.Analternatecatabolicnearlyconstantratio,almostidenticaltothatexistinghypothesisisbasedonthefindingthatpartiallydegradedlinkforthetotalchemicalamou

7、ntsofthesetwocomponentsproteinsaccumulateincartilagematrixwithincreasingageincartilagetissue;2)thesetwocomponentsarecatab-(10)andstatesthatproteolyticcleavageofthelinkproteinolizedwithvirtuallyidenticalkinetics;and3)thisinactivatesitsabilitytostabilizetheproteogl

8、ycan-hyaluron-catabolicrelationshipinvitroreflectsthelossofhy-aluronateandproteoglycansfromnative,undisso-atecomplex.Catabolismofthethirdcomponentofthecom-ciat