紫甘薯花色苷的组分及抗氧化活性研究

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1、万方数据第29卷第1期林产化学与工业v01．29No．12009年2月ChemistryandIndustryofForestProductsFeb·2009StudyonChemicalConstituentsandAntioxidantActivityofAnthocyaninsfromIpomoeabatatasL．(purplesweetpotato)ZHUHong-mei，ZHAOMeng(EngineeringCollege，ShanxiNormalUniversity，Linfen041004，China)Abstract：Anthocyaninsextractedfrom

2、purplesweetpotatoes(／po,,拼abammsL．)werepurifiedthmu#AB-8resincolumn．TheextractcontainsIkindsofanthocyaninsbyHPLC·DADanalysis．Themajorityoftheanthocyaninsareacylatedcyanidinandpeonidin．TheobjectiveofthisstudyWAgtoinvestigatetheZHUHong‘meiantioxidantactivitiesofanthoeyaninsfrompurplesweetpotatoesby

3、differentanalyticalmethods：theOr,a．diphenyl哥picrylhydrazyl(DPPH)radical-scavengingeffectandsuperoxideanionsradical-scavengingeffect，reducingpowerandantioxidanteffectOillipidperoxidationinalinoleicacidmodelsystem．Ataconcentrationof0．5s／L．thereducingpowerofanthocyanins，L-ascorbicacid(t,-aa)andbutyl

4、atedhydroxytoluene(BHT)reached0．572，0．460and0．121，respectively．Theresultdisplayedthatanthoeyaninspossessedexcellentreducingpower．Thefactthathalfinhibitionconcentrations(1C∞)ofanthocyaninsscavengingDPPHradicalandsupemxideanionsradicalwere6．94and3．68mg／Lrespectivelyindicatesthatantho-cyaninswereeff

5、ectiveinscavengingDPPHandsuperoxideanionsradical．Inaddition，枷ocy枷nscouldsignificantlyinhibittheperoxidationoflipid．Keywords：chemicalconstituent；antioxidantactivity；lpomoeabatasasL．(purplesweetpotato)；anmocyaIlin8CLCnumbers：TQ351．0Documentcode：AArtideⅢ：0253—2417(2009)Ol一0039—07紫甘薯花色苷的组分及抗氧化活性研究朱洪梅

6、，赵猛(山西师范大学工程学院，山西临汾041004)摘要：对紫甘薯花色苷的化学成分和抗氧化活性进行了研究。研究采用大孔树脂AB一8纯化紫甘薯花色苷，高效液相色谱一2极管阵列法(HPLC—DAD)分析表明，纯化后的提取物中共含有11种花色苷，其中主要成分为酰化的矢车菊素和芍药素。并测定了紫甘薯总花色苷在DPPH自由基清除体系、超氧阴离子体系、还原力和亚油酸体系的抗氧化活性。在质量浓度均为0．5g／L时，花色苷、卜从和BHT的还原力分别为0．572、0．460和0．121，花色苷的清除DPPH自由基的半数抑制浓度(Ic。)和清除超氧阴离子lc如分别为6．94和3．68ms／L，表明花色苷还原

7、能力强，并能有效地清除DPPH自由基和超氧阴离子。此外，紫甘薯花色苷能较好地抑制脂质过氧化。关键词：化学组分；抗氧化性；紫甘薯；花色苷Reactiveoxygenspecies(ROS)，includingsuperoxideanion(Of)，hydroxylradical-(OH)andhydrogenperoxide(H202)，existinlivingorganisms¨o．ROSformedduringnormalmet