牛磺酸对糖尿病大鼠膈肌酶活性的影响

《牛磺酸对糖尿病大鼠膈肌酶活性的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、维普资讯http://www.cqvip.com238JBengbuMedCoil，May2006，Vo1．31，No．3[文章编号]1000-2200(2006)03-0238-03·基础医学·牛磺酸对糖尿病大鼠膈肌酶活性的影响王蕾。关宿东[摘要]目的：观察牛磺酸对糖尿病(diabetesmelitus，DM)大鼠膈肌损伤的保护作用，探讨其作用机制。方珐：sD大风24只，随机分为正常对照组(NC)、糖尿病组(DM)、牛磺酸治疗组(Tau)。腹腔注射链脲菌素50mg／kg制备DM大鼠模型。Nc组和DM组予自来水

2、，Tau组予l％牛磺酸饮水，4周后测大鼠体重、血糖，检测膈肌组织中的超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)活性和丙二醛(MDA)、NO含量的变化；电镜下观察膈肌组织超微结构的改变。结果：与NC组相比。DM组大鼠膈肌组织中SOD、NOS活性、NO含量显著降低(P<0．01)，MDA含量显著增加(P<0．01)；电镜下，肌小节和线粒体形态改变。与DM组相比，Tau组SOD、NOS活性、NO含量增加(P<0．05和P<0．01)，血糖和MDA含量降低(P<0．01)；电镜下，超微结构损伤减轻。结论：牛磺酸可

3、提高DM大鼠膈肌组织中SOD、NOS活性和NO含量，降低血糖浓度、MDA含量，能保护DM膈肌的损伤。[关键词]糖尿病；膈肌；牛磺酸；酶；大鼠[中国图书资料分类法分类号]R578．1[文献标识码]AEfectsoftaurineonactivityofenzymesintissuesofdiaphragmindiabeticratsWANGLei，GUANSu—Dong(DepartmentofPhysiology，BengbuMedicalCollege，Bengbu233030，China)[Abstract

4、]Objectlve：Tostudytheefectsoftaurineondiaphragminjuryindiabeticrats．Methods：Twenty-fourSDratswererandomlydividedintonormalcontrol(NC)group，diabetic(DM)groupandtaurinetreatmenl(Tau)group．DMgroupandTaugroupratswel'egivenstreptozocin(STZ)50me：／kgintraperitoneal

5、injection．NCgroupandDMgroupratsweretreatedwithwatar，Taugroupratsweretreatedwith1％taurineindrinkingwatar．After4weeks，thebodyweight(BW)andlevelofbloodgheose(BG)wer'eme~umd．Supemxidedismutase(SOD)，nitricoxidesynthase(NOS)activitiesandmalondialdehyde(MDA)，NOcont

6、entsindiaphragmtissueswerealsomeasured．Theultrastructureofdiaphragmwereintvestigatedbyelectronmicroscope．Results：SOD，NOSactivities，NOcontentsweresignificantlylower(P<0．01)，BGandMDAcontentweresignificantlyhigherinDMgroup(P<0．01)．ComparewithDMgroup，SOD，NOSacti

7、vitiesandNOcontentswerehigher(P<0．05andP<0．01)，BGarAMDAcontentwerelowerinTaugroup(P<0．01)．TheuhrastructureunderelectronmicroscopeindicatedthatinDMgroupdiaphragmfibresfracture，mitochondrionsswellinganddegeneration．Theabovechangeswereinhibitedbytreatmentoftaur

8、ine．Conclusions：TaurinecanincreaseSOD，NOSactivities，NOcontentsanddecreaseMDAcontenindiabeticdiaphragm．[Keywords]diabetes；meUltus；diaphragm：taurine；enzyme；rats骨骼肌是胰岛素刺激状态下葡萄糖摄取的主要1材料与方法部位，并且骨骼肌又是