富硒螺旋藻清除氧自由基的初步研究

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1、营养学报2005年第27卷第1期61富硒螺旋藻清除氧自由基的初步研究彭赞3，黄峙‘,2郭宝江’(，华南师范大学生命科学学院，广州510631;2暨南大学生命科学技术学院，广州510632;，广州中医药大学基础医学院，广州510470)【摘要】目的与方法:采用化学发光法研究富硒螺旋藻提取液、硒藻蓝蛋白、硒藻多糖、亚硒酸钠对超氧阴离子(O豆)和Al自由基((-OH)的清除作用。绪果:当体系中提取液浓度达400gg/ml时，富硒比未富硒螺旋藻提取液对O互的清除率提高30%，对.-OH的清除率提高20%;硒藻蓝蛋白比藻蓝蛋

2、白对O互和.-OH的清除作用有所提高，并呈现明显的剂量相关性，O豆50%清除率蛋白浓度为500gg/ml,-OH50%清除率蛋白浓度为‘25gg/ml;硒藻多糖对氧自’由基具有极强的清除效率，O互和·OH50%清除率时的浓度分别为12gg/ml和60ug/ml;另外，在实验浓度范围，未检浏到亚硒酸钠时超O互和·OH具有清除作用。结论:富硒螺旋藻可能因硒的代谢而产生活性有机硒态如硒藻蓝蛋白、硒多糖等，从而提高其清除自由基活性。关键词:螺旋藻:硒;氧自由基中图分类号:R151.3文献标识码:A文章编号:0512-795

3、5(2005)01-0061-04THESCAVENGINGEFFECTSOFSEENRICHEDSPIRULINAPLATENS!SONOXYGENFREERADICALSPENGYun3，HUANGZhii'2，GUOBao一fang'('CollegeofLifeScience,SouthChinaNormalUniversity,Guangzhou510631;2CollegeofLifeScienceandBiotechnology,Ji势University,vuangznou.7iUw4;‘口“‘ge

4、uiBusemedicine,vuangznouUniverst尽o,/iraaauwnatMedicine,vuangznouJIU-r/Ui,nrnat[Abstract]ObjectiveandMethods:Thescavengingeffectsoftheextracts,phycocyaninandpolysaccharosefromSpirulinaplatensis(S.P)orfromseleniumenrichedS.P(Se-S.P),andsodiumseleniteonsuperoxide

5、anions(O豆)andhydroxylradicals(OH)wereinvestigatedrespectivelywithchemoluminescencetechnique.Results:Theextract,Se-phycocyaninandSe-polysaccharoeefromSe-S.PhadhigherscavengingeffectonbothkindsofactiveoxygenradicalsthanthosefromS.Pandthescavengingratesofextracto

6、nO互and-OHwereenhancedabout30%and20%ataconcentrationof400p,g/m1.ThoseeffectsofSe-phycocyaninwerecharacterizeddose-dependentlyandtheconcentrationof50%scavengingrateonsuperoxideanionswasabove500gg/ml.WithincreaseoftheconcentrationofSe-phycocyanin,thescavengingrat

7、eon-OHwaselevatedsteadilyand50%scavengingratewasfoundatproteinconcentrationabove125pg/ml.ThescavengingeffectoftheSe-polysaccharidesonoxygenfreeradicalswasmorenoticeableandtheconcentrationfor50%scavengingrateon-OHandO互were60gg/mland12gg/mlrespectively.However,t

8、herewasnoevidencedscavengingeffectofsodiumseleniteonboth-OHandO夏·Conclusion:TheactiveorganicSe-containingmoleculessuchasSe-containingproteinsandSepolysaccharides,whichwereproducedb