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1、582止塑6,Vol.27,No.12一一一一一一一AnalysisforFlavonoidsinBeebyCapillaryElectrophoresisLUTing'，EdwardPCLain,JornCCYu2,HUFeng-zu3(1.QinghaiInstituteofSupervisionandInspectiononProductQuality,Xining810008,China;2.Ottawa-CarletonChemistryInstitute,DepartmentofChemistry,CarletonUniversity,Ottawa,O
2、NKIS5B6,Canada;3.NorthwestPlateauInstituteofBiology,TheChineseAcademyofSciences,Xining810001,China)Abstract:Acapillaryelectrophoresis(CE)methodhasbeendevelopedforthedeterminationofsixbioactiveflavonoidsthatarecommonlyfoundinhealthfoods:hesperidin,hyperin,isorhamnetin,kaempferol,querce
3、tinandrutin.Theeffectsofseveralparameters,suchaspH,bufferconcentration,separationvoltageandUVdetectorwavelength,wereinvestigatedtofindtheoptimalconditions.UsingaH3BO3-Na2Ba0,buffer(pH9.2),theanalytescanbeseparatedwithin8min.Therelativestandarddeviationsofmigrationtimesineightinjection
4、swerebetween0.77%and0.93%,andthoseofthepeakareasrangedfrom3.8%to8.6%.Ahighreproducibilityandexcellentlinearitywasobservedovertwoordersofmagnitude,withdetectionlimits(S/N二3)rangingfrom0.34ugmlto2.9vgmlforallthesixanalytes.Recoveriesrangedfrom80.4%to113.9%.Thenewmethodissimple,reproduci
5、bleandsensitive.Nosolid户aseextractionforsamplepretreatmentisnecessary.Analysisresultsareaccurateinapplicationtobee,pollens.-Keywords:analysis;flavonoids;beepollen;capillaryelectrophoresis毛细管电泳法分析蜂花粉中黄叩类化合物卢挺’,EdwardPCLai2,JornCCYU2，胡风祖3(1.青海省产品质量监督检验所，青海西宁810008;2.卡尔顿大学化学系，加拿大涯太华一ONKI
6、S5B6;-3中国科学院西北高原生物研究所，青海西宁810001)摘要:使用毛细管电泳法(CE)检测了健康食品中常见的六种黄酮类化合物:橙皮贰、海棠贰、异鼠李素、山奈酚、棚皮素、芦丁。研究了一系列的试验参数，如:pH、缓冲溶液浓度、分离电压以及UV检测器波长等，以确定出最佳条件。使用H3BO3-Na2Be0?缓冲液((pH9.2)，各分析物可在8min内分出。相对标准偏差(RSD):8次进样的迁移时间为0.77%-0.93%;峰面积为3.8%-8.6%;各检测限((S/N=3)范围为0.3411g/ml^2.9ug/ml，回收率为80.4%一113.9%。方法简单
7、、灵敏，重现性高，线性好，无须固相萃取前处理，用于蜂花粉分析结果准确。关键词:分析;黄酮类化合物;蜂花粉;毛细管电泳中图分类号:0623.54「文献标识码:A文章编号:1002-6630(2006)12-0582-06Beepollenisafoodforyoungbees.Workerbeescollectlifespan.Beepollenprovestobequiteusefulforactivityflowerpollenswhicharethemalereproductivecells.Theye曲ancementandsportsnutrition.I
8、tprod
