尼罗罗非鱼鱼皮胶原蛋白中游离基清除肽的制备及其抗氧化活性

《尼罗罗非鱼鱼皮胶原蛋白中游离基清除肽的制备及其抗氧化活性》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、维普资讯http://www.cqvip.com第32卷第1期水产学报V01．32．No．12008年1月JOURNALOFFISHERIESOFCHINAJan．，2008文章编号：1000—0615(2008)01—0117—08PreparationofradicalscavengingpeptidesfromOreochromisniloticusskingelatinanditsantioxidativeactivityZENGMing—yong，GUOYao，LIUZun—ying，DONGShi—yuan，ZHAOYua

2、n—hui，CUIWen—xuan(CollegeofFoodScience，OceanUmversayofChina，咖266003，Ch／an)Abstract：Gelatinextractedfromtilapia(Oreochromisniloticus)skinwashydrolyzedwithserialdigestionsintheorderofBromelainandAlcalase．Theantioxidativeactivityinvitrooftilapiaskingelatinhydrolysates(TGH)

3、wasresearched．TheresultsshowedTGHhadstrongsuperoxide／hydroxylmdicalscavengingabilityandthevaluewas3．13mg。mL_。and3．58mg。mL_。．respectively．TheTGHwasfractionatedintofivemajortypesofTGH—I(>10ku)，TGH-Ⅱ(10—5ku)，TGH一111(5—3ku)，TGH-Ⅳ(3—1ku)，andTGH-V(below1ku)usinganultrafiltrat

4、ion(UF)membranebioreac~rsystembasedonthemolecularweightcut—offs．ThefractionofTGH-V，exhibitedthehighestsuperoxidescavengingactivity，wasfurtherpurifiedusingconsecutivechromatographicmethodsincludinggelfiltration，ion—exchangechromatography，andreverse—phasehighperformanceli

5、quidchromatography．Thepurifiedpeptidehadastrongscavengingabilityonsuperoxideandthevaluewas4．6ttg。mL～．Andthemolecularweightofthepurifiedpeptidewasbetween311．3—932．8udeterminedbyMS．Keywords：《)，BDc，D，nniloticus；gelatin；enzymichydrolysis；radicalscavengingpeptideCLCnumber：Q8

6、14Documentcode：AFreeradicalssuchassuperoxideanionradicalascompeptides[．soybeanpeptides【．caseinandhydroxylradicalcanreactwithmanysubstancespeptides[．pollackskinpeptidesandfermentedlikelipids，proteins，etc．Theyaleveryunstableandmusselsaucepeptides．possessantioxidativereact

7、rapidlywithothergroupsorsubstancesintheactivities．Thesepeptideswerecomposedof5to16body，leadingtocellortissueinjury¨．Theyplayanaminoacidresiduesandincludedhydrophobicaminoimportantroleinmanydiseasessuchascancer，acids(ValorLeu)attheN-terminus，andPro，Hisgastriculcersandart

8、hritis．Therefore，syntheticorTyrinsequences．Butthestructureofantioxidantantioxidantsalecommonlyusedtosolvesuchp