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1、JournalofGeneticsandGenomics(FormerlyActaGeneticaSinica)February2007,34(2):138-150ResearchArticleChitinasesinOryzasativassp.japonicaandArabidopsisthaliana①FenghuaXu,ChengmingFan,YueqiuHeKeyLaboratoryofPlantPathologyoftheMinistryofEducation,YunnanAgriculturalUniversity,Ku
2、nming650201,ChinaAbstract:Chitinases(EC3.2.1.14),foundinawiderangeoforganisms,catalyzethehydrolysisofchitinandplayamajorroleindefensemechanismsagainstfungalpathogens.Thealignmentandtypicaldomainswereanalyzedusingbasiclocalalignmentsearchtool(BLAST)andsimplemodulararchite
3、ctureresearchtool(SMART),respectively.Onthebasisoftheannotationsofrice(OryzasativaL.)andArabidopsisgenomicsequencesandusingthebio-softwareSignalP3.0,TMHMM2.0,TargetP1.1,andbig-PiPredic-tor,25outof37and16outof24openreadingframes(ORFs)withchitinaseactivityfromriceandArabid
4、opsis,respectively,werepredictedtohavesignalpeptides(SPs),whichhaveanaverageof24.8aminoacidsattheN-terminalregion.Someofthechitinasesweresecretedextracellularly,whereassomewerelocatedinthevacuole.Thephylogenicrelationshipwasanalyzedwith61ORFsand25knownchitinasesandtheywe
5、reclassifiedinto6clustersusingClustalXandMEGA3.1.Thisclassificationisnotcom-pletelyconsistentwhencomparedwiththetraditionalsystemthatclassifiesthechitinasesinto7classes.Thefrequencyofdistribu-tionofaminoacidresidueswasdistinctindifferentclusters.Thecontentsofalanine,glyc
6、ine,serine,andleucinewereveryhighineachcluster,whereasthecontentsofmethionine,histidine,tryptophan,andcysteinewerelowerthan20%.Eachclusterhaddistinctaminoacidcharacteristics.Alanine,valine,leucine,cysteine,serine,andlysinewererichinClustersⅠtoⅥ,respectively.Keywords:Chit
7、inase;rice;Arabidopsis;secretedcharacteristics;phylogeneticsChitinases(EC3.2.1.14),whicharepresentinexistsonlyinvacuolarchitinases.Somechitinasesvariousorganisms,catalyzethehydrolyticcleavageofhaveoneormorechitin-bindingdomains(CBD)fol-theβ-1,4-glycosidicbondinbiopolymer
8、sN-acetyl-lowingtheN-terminalsignalregion.Inaddition,thereglucosamineandarelargelyfoundinchitinofar-isa
