利用hplc建立油菜蜂花粉指纹图谱

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1、万方数据Ag“cu“u搿c‘encel&TechnoI嘿’201o’燃∞7_1喊138．AgriIturaIBasi—Sci扪⋯ndMeth—dCopyright2010nformationnstJtuteofAASrightsreservedcuITUrabaslc3clerceaOQ⑥。lJH．A¨IlneConstru('tionofFingerprintofRalPollenbyUsingonstructionofHPLC_ClerPrlapePoenbyUsnHAOXiao-111+。ZHOUYan-min92，ZHANGXin-y

2、ul1．TestingCenterofLiaoningAcademyofAgriculturalSciences，Shenyang110161；2．ShenyangAgriculturalUniversity。Shenyang110161AbstractTheflavonoidsisakindofcomplexcompoundandquercetinisamaincompositioninrapepollen．Takingquercetinasasampleandusingseparationtechnologyandhighperiorma

3、nce1iquidchromatography，chromatographyfingerprinttechniqueofChinesemedicineisintro-ducedinfoodfieldtoconstructchromatographyfingerprintofrapepollen，whichcanbeusedformedicinaluseandedibleuse．Themethodwillbecomeabeneficialsupplementforqualitycontrolmethodtoensurethesafetyande

4、ffectivenessofproducts．KeywordsHPLc：Rapepollen；Fingerprint；QuercatinThefingerprintisusinggraphicsmodetocharacterizeanddescribechemicaIinformationofChinesemedicinethroughmodernanalyticaltechniques．besides．itisacharacteristicspectrumofcomponentspopulationthroughspectrumorchro

5、-matographicdetermination。。．Thefingerprintcanoverallre-flectspeciesandquantityofchemicaIconstituentsinsample．besides．whentheactivecomponentsofsamplearenotde-cided．thefingerprintcandistinguishtruthandfalseandmoni-torsamplequality．Withthedevelopmentofchromatographictechnique，

6、becauseofsuperiorityofchromatographicfinger·print．ilIswidelyusedjnChinesemedicinefieldandbecomesanimportantChinesemedicinemeanofqualitycontrolofTra．ditionaIChinesemedicine．however．thistechnologyisseldomusedinfoodfieldatpresent．Thesize。colorandcompositionofrapepollenarediffe

7、r-entindifferentplants．however．theirinfluencesOnhumanarealsodifferent．ItconcentratesalInutrientsinplantkingdom．soitiscalledtheperfectfood．TherapebelongstoBrassicainCruciferae．therapepollencollectedbyworkerbeedoesnotonlycontainneedednutrientsofhuman．suchasprotein．fat，sugar．t

8、raceelementsandalIvitamins。butalsocontainsbioactivesubstancessuchasbio-flavonoid，c