血小板衍生生长因子对体外培养人椎间盘细胞功能的影响

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1、万方数据60中国临床康复第形卷舅2强磬2006—06一lO出版ChineseJournalofClinicalRehabilitation,June102006V01．JDNo．21血小板衍生生长因子对体外培养人椎问盘细胞功能的影响☆李小川，原泉，王艺璇，王海义·基础研究·中国医科大学附属第二临床医院骨科，辽宁省沈阳市110004李小川☆。男，1969年生，辽宁省沈阳市八，汉族，2004年中国医科大学毕业，博士，讲师，主要从事脊拄外科疾病的诊治研究。中图分类号：R318文献标识码：A文章编号：1671-59

2、26(2006)21—0060-03收稿日期：2005-11-18修回13期：2006-03-07(05—50-927588／S·S)Effectsofplatelet-derivedgrowthfactorsonthefunctionofhumanintervertebraldisccelIscultured／nvitroLiXiao．Chuan，YuanOuan，WangYi—xuan，WangHal·yiDepartmentofOrthopaedics．SecondClinicaiHospitalMf

3、iliatedtoChinaMedicalUniversity，Shenyang110004，LiaoningProvince，ChinaLiXiao—chun☆．Doctor,Lecturer，DepartmentofOrthopaedics．SecondClinicalHospitalAffiliatedtoChinaMedicalUniversity，Shenyang“0004．LiaoningProvince．ChinaReceived：2005—1l一18Accepted：2006-03一07Ab

4、stractAnd：Toobservetheeffectofplatelet．derivedgrowthfactorsfPDGFslonapoptosis，differentiationandproliferationaswellasmatrixsynthesisofhumanintervertebraldisccellculturedinvitro．METHoDS：TheexperimentwasconductedattheKeyLaboratoryofCongenitalMalformationsofH

5、ealthMinistryfromMaytoFebruary2004．EightpatientswhounderwentscoliosisanteriorspinalreleaseintheDepartmentofOrthopaedics，FirstClinicalHospitalAffiliatedtoChinaMedicalUniversitywererecruited．’nlerewasnometaboliccartilagediseaseintheexaminationbeforeoperation

6、．Theimervertebraldisctissuetakenoutduringtheoperation(informedconsentwasobtainedlwasimmediatelyputintheasepticculturebottlecontainingHAMF一12culturemedium．Humanintervertebraldisccellswereculturedinvitroinmonolayer．Thewell—grownintervertebraldiscceilsofsecon

7、dgenerationweretreatedwithPDGFsofdifierentconcentrations(1，10，100，1000I“g／L)．Apoptosisdetectionwasperformedwithterrainaldeoxynucleotidyltransferase—mediateddUTPnickend—labeling(TUNEL)．ThedifferentiationandProliferationofintervertebraldisccellsweredetectedw

8、ithflowcytometerandmethylthiazolyltetrazoliumfMTrlassay．ThelevelofhydroxyprolineandchondroitinsulfateinculturemediumWeredetectedwithchloraminesTmethodandAlcianmethod．RESULTS：(DTheeffectofPEGFsontheapoptosisof