豆豉中大豆异黄酮及苷元降血糖活性及其机理的研究

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1、维普资讯http://www.cqvip.com时珍国医国药2007年第18卷第7期LISHIZHENMEDICINEANDMATERIAMEDICARESEARCH2007VOL．18NO．7豆豉中大豆异黄酮及苷元降血糖活性及其机理的研究郭瑞华，霍文，刘正猛，吴少杰，王和平，王海龙，梁彦云(华北煤炭医学院，河北唐山063000)摘要：目的研究永川豆豉中大豆异黄酮及苷元的降血糖活性及其降血糖机制。方法永川豆豉粉碎，脱脂，然后用65％的乙醇浸泡，超声提取两次。所得提取液离心后旋转蒸发，用无水乙醇提取可

2、得大豆异黄酮的粗提品。正丁醇饱和的水溶液除糖及色素等。然后经硅胶分离出大豆黄素和染料木素。河南鼠注射四氧嘧啶造成高血糖模型，连续7d灌胃给予所提取的大豆异黄酮及苷元。用葡萄糖氧化酶法测定血糖水平。结果永川豆豉中的大豆异黄酮及苷元能显著地降低四氧嘧啶糖尿病模型鼠血糖水平，缓解糖尿病症状；大豆异黄酮和染料木素都能抑制葡萄糖的活性，尤以染料木素最为明显。结论豆豉中的大豆异黄酮可以明显地降低小鼠的高血糖，其所含的成分染料木素为主要的降血糖成分之一。关键词：豆豉；大豆异黄酮及苷元；一葡萄糖苷酶；染料木素；降血

3、糖中图分类号：R285．5文献标识码：A文章编号：1008-0805(2007)07—1606-02StudyonHypoglycemicActivityandFunctionalMechanismofSoybeanIsoflavoneGly。coneofLobsterSauceGUORui—hua，HUOWen，LIUZheng—meng，wuShao~ie，WANGHe-ping，WANGHai-long，LIANGYah—yun(NorthChinaCoalMedicalCollege，Tan

4、gshan063000，Cina)Abstract：ObjectiveThisarticlestudiedtheactivitiesandmechanismofYongehuanfermentedsoybeanisoflavoneglyeonetore-ducethelevelofbloodsugar．MethodsAftergroundandremovedofoil．Yongehuanfermentedsoybeanwasultrasonicextractedwith65％alcoholtwice

5、．TheextractionWascentrifuged．TheliquidWasrotateevaporatedandthenextractedwithalcoholtoobtainthecrudeextract．Thiscrudeextractionwasremovedofsugarandpigmentwithbutanolsaturatedwaterandwasseparatedbysilicagelchromatographytoyielddadezeinandgenestein．Highb

6、loodsugarmodelWasconstructedbytheinjectionofalloxanintoHenanmice．Themiceweretreatedwithsoyaisoflavonesforcontinuous7days．ThebloodsugarlevelWasmeasuredbyGOD(glucoseoxidase)method．ResultsThesoyaisoflavoneglyeonefromYongchuanfermentedsoybeancanremarkabler

7、educethelevelofbloodsugarcausedbyalloxan，relaxingthesymptomsofdiabetes．BothcrudeextractofsoyaisoflavonesfromYongchuanfermentedsoybeanandgenesteinhadtheabilitytoinhibittheactivityof—glueosidase．withgenesteinmorepotent．ConclusionThesoyaisoflavonesandisof

8、lavoneglyconefromYongchuanfermentedsoybeancanobviouslyreducethehype叫ycemiaofalloxanmiceandthemaincomponentofreducingbloodsugarmayduetogenestein．Keywords：soybeanisoflavoneglyeone；—glyeosidase；Genestin；Reducingactivityonbloodsugar豆豉在我国有着悠