小檗碱抑制兔胆固醇结石形成的作用和机制.doc

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1、小檗碱抑制兔胆固醇结石形成的作用和机制作者：王江华　陈先祥　蔡庆和　程彩涛　何志军　吴黎明【摘要】　　目的观察小檗碱对兔胆囊胆固醇结石形成的预防作用和机制。方法动物模型：日本大耳白兔30只，随机等分三组：每组10只，对照组喂普通饮食；结石组，喂含1.2%高胆固醇饲料100g；实验组，同时喂饲含1.2%高胆固醇饲料100g和每日10mg/kg的小檗碱灌胃。8周后利用全自动生化分析仪酶法测定兔胆汁中的总胆固醇、磷脂、胆盐，通过Carey表计算胆固醇饱和指数(cholesterolsaturationindex，CSI)，观察各组胆囊胆

2、汁结晶情况及环氧合酶-2(cyclooxygenase2，COX2)含量。结果实验组比成石组形成胆固醇结晶机会明显减少(8／10、3／10，?字2=5.051，P<0.05)；各组胆囊胆汁中CSI(对照组0.82±0.23、结石组1.64±0.27及实验组1.13±0.29)；结石组COX2表达与空白组相比明显升高(47.66±1.54比28.39±1.46，P<0．05)，实验组较之结石组COX2表达量有降低(37.42±2.45比47.66±1.54，P<0.05)。结论小檗碱能够抑制胆固醇结石形成和发展，其

3、机制与调节脂质代谢，改善致石性胆汁及抑制COX2的活性等作用有关。【关键词】胆固醇结石；盐酸小檗碱；环氧合酶-2；兔　　InhibitoryeffectsofberberineontheformationofcholesterolgallstoneanditsmechanisminrabbitsAbstractObjectiveToinvestigateandobservetheinhibitoryeffectsofberberineontheformationofexperimentalgallstoneandexploreit

4、spossiblemechanism．MethodsThreedietarygroupsofJapaneserabbitswith10rabbitsineachgroupwereallocatedascontrol（fedwithnormaldiet），lithogenic(fedwith1.2ghighcholesterol)andberberineprevention(fedwith1.2ghighcholesteroldiet+10berberinemg/kg/day)groupsrespectivelyfor8weeks.

5、CheckthelevelofTC，PL，BS，bilewasobtainedforobservinggallstoneformation，andthemorphologicchangesofthemucosawereassessedunderthemicroscope.ThelevelofCOX2ingallbladdermucosaineachgroupwasestimatedwithimmunohistochemicalassay.ResultsTheopportunityoftheformationofthecholest

6、erolcrystallintheberberinegroupwasobviouslylessthanthoseingallstonegrouptoformgallstone(3/10vs8/10，P<0.05).CSIineachgroupwasasfollow：(control0.82±0.23，lithogenic1.64±0.27andberberineprevention1.13±0.29)，respectively.ThedegreeofmucosalinflammationandlevelofCOX2expre

7、ssionweresignificantlyhigheringallstonegroupthanthatintheothertwogroups(47.66±1.54vs28.39±1.46，P<0.05；37.42±52.45vs47.66±1.54，P<0.05).ConclusionsBerberinecaninhibittheformationanddevelopmentofcholesterolgallstone．Itsmechanismisrelatedtotheadjustmentoflipidmetabo

8、lism,improvementoflithogenicbileandinhibitionoftheactivityofCOX-2.Keywordscholesterolgallstone；berberine；cyclooxygenase2；rab