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ID:1769688
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页数:7页
时间:2017-11-13
《医药学临床医学毕业论文 tpa对人食管癌eca109细胞增殖和细胞核形态的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、湖南师范大学本科毕业论文考籍号:XXXXXXXXX姓名:XXX专业:医药学临床医学论文题目:TPA对人食管癌Eca109细胞增殖和细胞核形态的影响指导老师:XXX二〇一一年十二月十日作者:郭玉忠,贾琳静,郜娜,田鑫,谢敏,张莉蓉,乔海灵【摘要】目的研究佛波酯(12-O-tetradecanoylphorbol-13-acetate,TPA)对人食管癌细胞的影响。方法细胞增殖试验采用MTT法和细胞计数的方法,细胞核形态的测量采用计算机辅助的图像分析系统,分别测量计算细胞核面积(NA)、核周长(NP)、最大直径(Dmax)、最小直径(Dmin)、核浆比(NPA)、核轴比(NAR)、核形状
2、因子(NRF)等。结果TPA没有抑制人食管癌Eca109细胞增殖和改变细胞核形态。结论TPA对人食管癌Eca109细胞可能没有促分化作用。【关键词】佛波酯;食管癌细胞;细胞核形态EffectsofTPAonproliferationandnuclearmorphometryofhumanesophagealcancerEca109cells【Abstract】ObjectivesToinvestigatetheeffectsof12-O-tetradecanoylphorbol-13-acetate(TPA)onhumanesophagealcancerEca109cells.Met
3、hodsThesensitivityofTPAtoEca109cellswasdeterminedwithMTTassayandcountingcellnumbers.AfterthecellswerestainedwithHE,themeasurementsofnuclearmorphometrywereperformedwithcomputer-assistedimageanalysissystem.ResultsTPAdidnotinhibitthecellsproliferationanddecreasethenuclearsize.ConclusionsTPAmaynoti
4、nduceEca109cellstodifferentiate.【Keywords】12-O-tetradecanoylphorbol-13-acetate(TPA);esophagealcancercellline;nuclearmorphometryHumanesophagealcancerisoneofthemostcommonsolidtumorsinChina.Duetoitshighmetastaticpotentialandthefrequentonsetofresistancetochemotherapy,itisoneofthemajorcausesofdeathb
5、yneoplasiainthecountry.Thepresentchemotherapyofcancerusesagentsthatareusuallytoxictonormalcells.Ontheotherhand,inductionofcellulardifferentiationmaysupplementtheuseofnon-cytotoxicdrugsinseveralformsofneoplasia,likethesuccessfuluseofall-transretinoicacid(ATRA)inthetreatmentofacutepromyelocyticle
6、ukemia.Plantditerpinephorbolesters,particularlythephorbolester12-O-tetradecanoylphorbol-13-acetate(TPA),areamongthemostpotenttumorpromotersknownandtheireffectsrangefromstimulatingDNAsynthesisthroughactivatinggeneexpressiontoproducingalterationsinmembranepropertiesandconstituents.TPAcanalsopoten
7、tiatethedifferentiationofvarioustumorcellsincultureandinvivoofhumanbeings.TPAcaninducedifferentiationandinhibitthegrowthofanumberofmalignantcelltypes,includingmyeloidleukemia[1],breast[2],prostate[3],colon[4],andbrain[5].Otherrepo
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