TPA对人食管癌Eca109细胞增殖和细胞核形态的影响.doc

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1、TPA对人食管癌Eca109细胞增殖和细胞核形态的影响作者:郭玉忠，贾琳静，郜娜，田鑫，谢敏，张莉蓉，乔海灵【摘要】目的研究佛波酯(12-O-tetradecanoylphorbol-13-acetate，TPA)对人食管癌细胞的影响。方法细胞增殖试验采用MTT法和细胞计数的方法，细胞核形态的测量采用计算机辅助的图像分析系统，分别测量计算细胞核面积(NA)、核周长(NP)、最大直径(Dmax)、最小直径(Dmin)、核浆比(NPA)、核轴比(NAR)、核形状因子(NRF)等。结果TPA没有抑制人食管癌Eca109细胞增殖和改变细胞核形态。结论TPA对人食管癌Eca109细胞可能没有促分化作

2、用。【关键词】佛波酯；食管癌细胞；细胞核形态EffectsofTPAonproliferationandnuclearmorphometryofhumanesophagealcancerEca109cells【Abstract】ObjectivesToinvestigatetheeffectsof12-O-tetradecanoylphorbol-13-acetate(TPA)onhumanesophagealcancerEca109cells.MethodsThesensitivityofTPAtoEca109cellswasdeterminedwithMTTassayandcounti

3、ngcellnumbers.AfterthecellswerestainedwithHE，themeasurementsofnuclearmorphometrywereperformedwithcomputer-assistedimageanalysissystem.ResultsTPAdidnotinhibitthecellsproliferationanddecreasethenuclearsize.ConclusionsTPAmaynotinduceEca109cellstodifferentiate.【Keywords】12-O-tetradecanoylphorbol-13-ac

4、etate(TPA)；esophagealcancercellline；nuclearmorphometryHumanesophagealcancerisoneofthemostcommonsolidtumorsinChina.Duetoitshighmetastaticpotentialandthefrequentonsetofresistancetochemotherapy，itisoneofthemajorcausesofdeathbyneoplasiainthecountry.Thepresentchemotherapyofcancerusesagentsthatareusuall

5、ytoxictonormalcells.Ontheotherhand，inductionofcellulardifferentiationmaysupplementtheuseofnon-cytotoxicdrugsinseveralformsofneoplasia，likethesuccessfuluseofall-transretinoicacid(ATRA)inthetreatmentofacutepromyelocyticleukemia.Plantditerpinephorbolesters，particularlythephorbolester12-O-tetradecanoy

6、lphorbol-13-acetate(TPA)，areamongthemostpotenttumorpromotersknownandtheireffectsrangefromstimulatingDNA5synthesisthroughactivatinggeneexpressiontoproducingalterationsinmembranepropertiesandconstituents.TPAcanalsopotentiatethedifferentiationofvarioustumorcellsincultureandinvivoofhumanbeings.TPAcani

7、nducedifferentiationandinhibitthegrowthofanumberofmalignantcelltypes，includingmyeloidleukemia［1］，breast［2］，prostate［3］，colon［4］，andbrain［5］.OtherreportshowedthatTPAcouldnotinducehumanhepatocarcinomacelllinetodif