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1、灵芝三萜类化合物对AD模型大鼠脑组织中CAT,ACHE,SOD,MDA的影响【摘要】 目的：探讨灵芝三萜类化合物（GLT）对阿尔茨海默病(AD)的防治作用，为临床新药的开发和应用提供理论依据。方法:将经Morris水迷宫筛选合格的SD大鼠80只分为8组，正常对照组、假手术组,模型组、GLT低、中、高（GLT0.25、0.5、1.0g/kg)剂量组、阳性药组（健脑胶囊组0.54g/kg）和溶媒组（食用油10ml/kg）,除正常对照组和假手术组外，其余各组大鼠经侧脑室注射Aβ25-352μl制备AD大鼠模型，正常对照组侧脑室注射等量生理盐水，术后次日开始
2、灌胃给药。每天1次，连续给药20d后，处死动物取脑组织制备匀浆。采用光化学法检测脑匀浆中过氧化氢酶（CAT）、乙酰胆碱酯酶（ACHE）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果:与正常对照组比较，模型组脑组织中CAT活性降低，ACHE活性升高，SOD活性降低，MDA含量升高。与模型组比较，三个GLT给药组脑组织中，CAT活力均升高，ACHE活力均下降，SOD活性均升高，MDA含量均降低。结论:灵芝三萜类化合物对AD模型大鼠具有增强清除自由基的能力，抗脂质过氧化及促进胆碱能神经系统功能的恢复。【关键词】阿尔茨海默病；灵芝属；脑；过氧化氢酶
3、；乙酰胆碱脂酶； ［Abstract］Objective:Toinvestigatethepreventiveeffectsofganodermolucidumtriterpenoids(GLT)toAlzheimerdisease（AD）inmodelrats.soastoprovidetheorybasisfornewdrugexploitationandclinicalapplication.Methods:EightySDratschosenbyMorriswatermazetestwererandomlydividedinto8groups
4、:normalcontrolgroup(groupNC,received10ml/kgofdistilledwater),shamoperationgroup(groupSO,received10ml/kgofdistilledwater),modelgroup(groupM,received10ml/kgofdistilledwater),GLTtreatedgroups(groupG1,received0.25g/kgofGLT;groupG2,0.50g/kg,andgroup3,1.0g/kg),masculinedruggroup(group
5、MD,received0.54g/kgofJiannaocapsules)，andsolutemediatedgroup(groupSM,received10ml/kgofedibleoil).AlloftheSDratsexceptthoseingroupNCandgroupSOweremicroinjectedwithbeta-amyloidpeptidesegment25-35(Aβ25-35)intolateralventricletoproduceADmodel,andinstead,ratsingroupNCwereinjectedwith
6、saline.GLTtreatmentbeganonthenextday,onceadayintragastricallyfor20days.Then,theanimalswereexecuted,andthebraintissuesweretakenoutandblendedforthetestsofactivitiesofcatalase(CAT),acetylcholinesterase(ACHE),superoxidedismutase(SOD),andthecontentofmalondialdehyde(MDA)withchemistryd
7、etectingkits.Results:TheactivitiesofCAT,SODwerelower,whileACHEactivityandMDAcontentwerehigherinratsofgroupMthanthoseingroupNC;TheactivitiesofCAT,SODwerehigher,whileACHEactivityandMDAcontentwerelowerinGLTgroupsthan5thoseingroupM.Conclusion:TheGLThasabilityofstrengtheningthecleani
8、ngoffreeradicalsinAβ25-35toxicatedrats,andabili
