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1、不同地区桔梗多糖含量的比较张小斌(商洛学院生物医药工程系、中国中医研究院商洛中药材GAP科研工程中心陕西商洛726000)摘要选取陕西商洛、安徽太和和吉林延边产桔梗,采用水提取法提取桔梗粗多糖,分别测定粗多糖含量,采用三氯乙酸-正丁醇法脱蛋白脱去多糖中粗蛋白,测定精制多糖含量,比较三个地区桔梗粗多糖及精制多糖含量。结果显示,安徽太和粗多糖含量最高,为26.36%,其次为吉林延边桔梗,为18.27%,陕西商洛桔梗为17.08%。提取粗多糖经精制后,安徽太和、陕西商洛、吉林延边桔梗粗多糖蛋白去除率分别为:74.27%,68.08%和69.36%,差异不显著。精制多糖含量分别为:12
2、.61%,15.42%和11.42%。结果表明安徽太和桔梗多糖含量最高,陕西商洛次之,最后为吉林延边桔梗。关键词:桔梗;粗多糖;精制多糖;含量中图分类号:文献标识码:文章编号:ComparisonthecontentofpolysaccharideofplatycodoninthreeareasAbstract:Selecttheplatycodon,inshangluo,anhuitaiandjilininyanbianwithwatertoextracthecrudepolysaccharideofplatycodon,ahdthencrudepolysaccharidei
3、sdetermined,Usetheacid-n-buohthedifferentmethodtotakeofftheproteininthepolysaccharidecrude,andthentodeterminetherefinedpolysaccharide,comparethethickpolysaccharidesandrefinedpolysaccharideofplatycodoninthesethreeareas.Theresultisshowedthatthehighestcontentofpolysaccharideinanhuitai,thickfor2
4、6.36%,followedbyjilinyanbianballoonflower18.27%,theminimumisshangluoballoonflower,for17.08%..Aftertheextractionofrefinedpolysaccharidefromcrudepolysaccharide,theballoonflowerthickglycoproteinremovalrateinanhuitai,shangluo,jilinyanbianismorethanrespectively74.27%,68.08%and69.36%,thedifference
5、isnotsignificant.Refinedpolysaccharideis12.61%,15.42%and11.42%,theresultsshowthatthecontentsofpolysaccharideinanhuitaiballoonflowerhighest,inshangluoisthesecondsmallest,andfinallyforjilinyanbianlisianthus.Keywords:platycodon;crudepolysaccharide;Refinedpolysaccharide;contentlocatedintheTomb,D
6、ongShenJiabang,deferthenextdayfocusedontheassassination.Linping,Zhejiang,1ofwhichliquorwinemasters(WuzhensaidinformationisCarpenter),whogotAfewbayonets,duetomissedfatal,whennightcame1.1选题的目的桔梗[1](Platycodongrandiflorum(Jacq.)A.DC.)是桔梗科桔梗属、多年生草本植物,别名铃铛花、梗草、和尚头花、土人参、包袱花等。药用其根,为桔梗科植物桔梗的干燥根。我国最早
7、药书《神农本草经》有记载,桔梗系常用中药桔梗花大美丽,可供观赏,种子可榨油,根是一种常见的中药材。其味苦、辛,性微温有宣肺、散寒、祛痰、排脓等功效,主治外感咳嗽、喉痹咽痛、胸满肋痛等症。现代医学认为,桔梗富含桔梗皂甙、多糖、甾醇类、萜类、桔梗酸等成分,并含有十几种氨基酸及铜、铁、锌、锰等22种微量元素,具有抗炎、止咳、化痰、抗溃疡、扩张血管、降血压、解热镇痛、镇静、降血糖、促进胆酸分泌、抗过敏等许多药理及保健功能[2]。除此以外,桔梗还可以作为蔬菜食用,在朝鲜、韩国、日本及我国东北地区,人们